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Phylogenetic diversity of epibiotic bacteria in the accessory nidamental glands of squids (Cephalopoda: Loliginidae and Idiosepiidae)

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Abstract

Bacterial communities were identified from the accessory nidamental glands (ANGs) of European and Western Pacific squids of the families Loliginidae and Idiosepiidae, as also in the egg capsules, embryo and yolk of two loliginid squid species, and in the entire egg of one idiosepiid squid species. The results of phylogenetic analyses of 16S RNA gene (rDNA) confirmed that several phylotypes of α-proteobacteria, γ-proteobacteria and Cytophaga–Flavobacteria–Bacteroides phylum were present as potential symbiotic associations within the ANGs. Several identified clones were related to reference strains, while others had no known close relatives. Gram positive strains were rare in loliginid squids. Several bacterial groups may play important roles in the function of the ANGs, such as production of the toxic compounds involved in egg protection and carotenoid pigments. Within the eggs, no bacteria were associated with embryo or yolk of Loligo vulgaris and Sepioteuthis lessoniana, but α- and γ-proteobacteria were present in the egg capsules. Most bacterial strains detected in the egg capsules were the same as those found in the ANGs. The cephalopods of temperate regions (European cuttlefishes and the squid L. vulgaris) appear to be associated with one Agrobacterium strain (Agro2) while tropical-subtropical strains (Asian and Australian loliginids) are associated with Silicibacter-related strains, suggesting a biogeographic clustering for the Agrobacterium-like strains.

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Acknowledgements

The authors wish to thank: Dr. C.C. Lu for his help during collection of specimens in Taiwan and for providing Loliolus samples; Dr. J. Nabhitabhata for Idiosepius biserialis egg samples from Thailand; J. Finn for assistance in field collection in Australia; Dr. H. Rybarczyk for assistance in statistical analysis. This work was supported by a CNRS grant (PICS 743) which provided 3 months salary and funds for field collection in Australia for the second author. The experiments complied with the current laws of the country in which the experiments were performed.

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Authors and Affiliations

  1. Département Milieux et Peuplements aquatiques (MNHN), Biologie des Organismes marins et Ecosystème (UMR5178, CNRS), 55 rue Buffon, 75005, Paris, France

    Delphine Pichon & Renata Boucher-Rodoni

  2. Cantonal Institute of Microbiology, University of Geneva, 6500, Bellinzona, Switzerland

    Valeria Gaia

  3. Museum Victoria, GPO Box 666E, Melbourne, 3001, VIC, Australia

    Mark D. Norman

Authors
  1. Delphine Pichon
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  2. Valeria Gaia
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  3. Mark D. Norman
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  4. Renata Boucher-Rodoni
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Correspondence to Delphine Pichon.

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Communicated by S.A. Poulet

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Pichon, D., Gaia, V., Norman, M.D. et al. Phylogenetic diversity of epibiotic bacteria in the accessory nidamental glands of squids (Cephalopoda: Loliginidae and Idiosepiidae). Marine Biology 147, 1323–1332 (2005). https://doi.org/10.1007/s00227-005-0014-5

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