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Preparation of diosgenin from Dioscorea zingiberensis C. H. Wright by stepwise biocatalysis-foam separation-preparative high-performance liquid chromatography (P-HPLC)

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Abstract

A novel method of stepwise biocatalysis-foam separation-preparative high-performance liquid chromatography (P-HPLC) to extract dioscin of dioscorea zingiberensis. C. H. Wright (DZW) was presented. Compared with the traditional process of direct hydrolysis of DZW, this novel process reduced the non-saponins material entering the hydrolysis, and, as a result, reduced the consumptions of water, acid, and organic solvent significantly, while simultaneously increasing the saponin yield. In addition, the P-HPLC was more efficient, which can not only get high purity, but also the product yield, high speed, simple operation, and it is convenient for industrialized production preparation.

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Acknowledgements

This research was financially supported by 2017 basic research project Qinghai, China (No. 2017-ZJ-712). The authors thank Prof. Qixiu ZHU of the Key Laboratory of functional organic molecules in Lanzhou University for the assistance in 1HNMR experiments, and Yaling Chi of the Qinghai Normal University for assistance in GC-MS experiments.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Qinghai Normal University, Xining, 810008, China

    Lei Lei, Wei Zhang, Zhongchao Gao, Long Liu & Xiaodong Yu

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  1. Lei Lei
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  2. Wei Zhang
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  3. Zhongchao Gao
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  4. Long Liu
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  5. Xiaodong Yu
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Correspondence to Wei Zhang.

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All authors declare that we have no conflict of interest, and the article is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part.

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This article does not contain any studies with human or animal subjects.

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Lei, L., Zhang, W., Gao, Z. et al. Preparation of diosgenin from Dioscorea zingiberensis C. H. Wright by stepwise biocatalysis-foam separation-preparative high-performance liquid chromatography (P-HPLC). Eur Food Res Technol 244, 1447–1452 (2018). https://doi.org/10.1007/s00217-018-3058-8

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  • DOI: https://doi.org/10.1007/s00217-018-3058-8

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