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Authentication of the most important species of rockfish by means of fins

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Abstract

In the present work, a method for the authentication of more of 100 scorpaenids species has been developed by means of forensically informative nucleotide sequencing technique, which is based on a PCR followed by a phylogenetic analysis. Due to the different commercial value of the species belonging to this taxonomic group, substitutions between species in seafood products can take place. Two different methodological strategies are proposed, allowing the analysis of fresh, frozen, or highly processed products with total reliability. These analytical systems have been validated and subsequently applied to 25 commercial samples. Therefore, this technique can be used as a routine method to avoid the mislabeling in the marketing of scorpaenid species and it is also suitable to assess the correct seafood traceability of these products.

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References

  1. Amidei RE (1986) Rockfish, a focus for research?: proceedings of a California Sea Grant Workshop. California Sea Grant College Program, Institute of Marine Resources, University of California

  2. Love MS, Yoklavich M, Thorsteinson LK (2002) The rockfishes of the northeast Pacific. University of California Press, Berkeley

    Google Scholar 

  3. Fishbase. http://www.fishbase.org/search.php

  4. Kim IC, Lee JS (2004) The complete mitochondrial genome of the rockfish Sebastes schlegeli (Scorpaeniformes, Scorpaenidae). Mol Cells 17:322–328

    CAS  Google Scholar 

  5. FAO. http://www.fao.org/index_es.htm

  6. IUCN Red List. The IUCN red list of threatened species: http://www.iucnredlist.org

  7. Alesandrini S, Bernardi G (1999) Ancient species flocks and recent speciation events: what can rockfish teach us about cichlids (and vice versa)? J Mol Evol 49:814–818

    CAS  Google Scholar 

  8. Johns GC, Avise JC (1998) Tests for ancient species flocks based on molecular phylogenetic appraisals of Sebastes rockfishes and other marine fishes. Evolution 52:1135–1146

    Article  Google Scholar 

  9. Kai Y, Nakayama K, Nakabo T (2003) Molecular phylogenetic perspective on speciation in the genus Sebastes (Scorpaenidae) from the Northwest Pacific and the position of Sebastes within the subfamily Sebastinae. Ichthyol Res 50:239–244

    Article  Google Scholar 

  10. Kendall AW (2000) An historical review of Sebastes taxonomy and systematics. Mar Fish Rev 62:1–23

    Google Scholar 

  11. Rocha-Olivares A, Kimbrell CA, Eitner BJ, Vetter RD (1999) Evolution of a mitochondrial cytochrome b gene sequence in the species-rich genus Sebastes (Teleostei, Scorpaenidae) and its utility in testing the monophyly of the subgenus Sebastomus. Mol Phylogenet Evol 11:426–440

    Article  CAS  Google Scholar 

  12. Magnuson-Ford K, Ingram T, Redding DW, Mooers AO (2009) Rockfish (Sebastes) that are evolutionarily isolated are also large, morphologically distinctive and vulnerable to overfishing. Biol Conserv 142:1787–1796

    Article  Google Scholar 

  13. Seeb LW (1998) Gene flow and introgression within and among three species of rockfishes, Sebastes auriculatus, S-caurinus, and S-maliger. J Hered 89:393–403

    Article  Google Scholar 

  14. Rocha-Olivares A (1998) Multiplex haplotype-specific PCR: a new approach for species identification of the early life stages of rockfishes of the species-rich genus Sebastes Cuvier. J Exp Mar Biol Ecol 231:279–290

    Article  CAS  Google Scholar 

  15. Rocha-Olivares A (1998b) Molecular evolution, systematics, zoogeography and levels of intraspecific genetic differentiation in the species of the antitropical subgenus Sebastomus, Sebastes (Scorpaeniformes, Teleostei) using mitochondrial DNA sequence data. University of California, San Diego

  16. Rocha-Olivares A, Rosenblatt RH, Vetter RD (1999) Cryptic species of rockfishes (Sebastes:Scorpaenidae) in the Southern Hemisphere inferred from mitochondrial lineages. J Hered 90:404–411

    Article  CAS  Google Scholar 

  17. Rocha-Olivares A, Rosenblatt RH, Vetter RD (1999) Molecular evolution, systematics, and zoogeography of the rockfish subgenus Sebastomus (Sebastes, Scorpaenidae) based on mitochondrial cytochrome b and control region sequences. Mol Phylogenet Evol 11:441–458

    Article  CAS  Google Scholar 

  18. Rocha-Olivares A, Vetter RD (1999) Effects of oceanographic circulation on the gene flow, genetic structure, and phylogeography of the rosethorn rockfish (Sebastes helvomaculatus). Can J Fish Aquat Sci 56:803–813

    Article  Google Scholar 

  19. Bentzen P, Wright JM, Bryden LT, Sargent M, Zwaneburg KCT (1998) Tandem repeat polymorphism and heteroplasmy in the mitochondrial control region of redfishes (Sebastes:Scorpaenidae). J Hered 89:1–7

    Article  CAS  Google Scholar 

  20. Roques S, Duchesne P, Bernatchez L (1999) Potential of microsatellites for individual assignment: the North Atlantic redfish (genus Sebastes) species complex as a case study. Mol Ecol 8:1703–1717

    Article  CAS  Google Scholar 

  21. Herrero B, Vieites JM, Espiñeira M (2011) Duplex real-time PCR for authentication of anglerfish species. Eur Food Res Technol 233:817–823

    Article  CAS  Google Scholar 

  22. Winfrey MR, Rott MA, Wortman AT (1997) UnraVeling DNA: molecular biology for the laboratory. Prentice Hall, New York

    Google Scholar 

  23. Burgner M, Hübner P (1998) Mitochondrial DNA enrichment for species identification and evolutionary analysis. Eur Food Res Technol 207:261–263

    Google Scholar 

  24. Mc Carthy C (1996) Chromas version 1.45. School of Health science, Griffifth University, Gold Coast Campus, Queensland, Australia

  25. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  Google Scholar 

  26. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  27. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  Google Scholar 

  28. Saitou N, Nei M (1987) The neighbor-joining method—a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  29. Espiñeira M, Gonzalez-Lavin N, Vieites JM, Santaclara FJ (2009) Development of a method for the identification of scombroid and common substitute species in seafood products by FINS. Food Chem 117:698–704

    Article  Google Scholar 

  30. Espiñeira M, Vieites JM, Santaclara FJ (2009) Development of a genetic method for the identification of salmon, trout, and bream in seafood products by means of PCR–RFLP and FINS methodologies. Eur Food Res Technol 229:785–793

    Article  Google Scholar 

  31. Espiñeira M, Vieites JM, Santaclara FJ (2010) Species authentication of octopus, cuttlefish, bobtail and bottle squids (families Octopodidae, Sepiidae and Sepiolidae) by FINS methodology in seafoods. Food Chem 121:527–532

    Article  Google Scholar 

  32. Lago FC, Herrero B, Vieites JM, Espiñeira M (2011) FINS methodology to identification of sardines and related species in canned products and detection of mixture by means of SNP analysis systems. Eur Food Res Technol 232:1077–1086

    Article  CAS  Google Scholar 

  33. Lago FC, Vieites JM, Espiñeira M (2012) Authentication of the most important species of freshwater Eels by means of FINS. Eur Food Res Technol 234(4):689–694

    Google Scholar 

  34. Santaclara FJ, Espineira M, Vieites JM (2007) Genetic identification of squids (Families Ommastrephidae and Loliginidae) by PCR-RFLP and FINS methodologies. J Agric Food Chem 55:9913–9920

    Article  CAS  Google Scholar 

  35. Bartlett SE, Davidson WS (1992) Fins (forensically informative nucleotide sequencing)—a procedure for identifying the animal origin of biological specimens. Biotechniques 12:408–411

    CAS  Google Scholar 

  36. Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial-DNA in humans and chimpanzees. Mol Biol Evol 10:512–526

    CAS  Google Scholar 

  37. Hillis DM, Bull JJ (1993) An empirical-test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst Biol 42:182–192

    Google Scholar 

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Acknowledgments

We thank Fátima C. Lago and Antonio Gavalás for their helpful assistance. We also thank to Vera Sequeira (University of Lisbon, Portugal), Jose Antonio González Pérez (Canary Institute of Marine Science, Spain), José Manuel Bautista (Complutense University of Madrid, Spain), Paloma Morán (University of Vigo, Spain), Sarah Lyon (University of Alaska Fairbanks, Alaska), Malcolm Wyeth (Pacific Biological Station, Nanaimo), Mark Tagal (National Oceanic and Atmospheric Administration, NOAA), Kaj Sünksen (Greenland Institute of Natural Resources, Greenland), Jan Yde Poulsen (University of Bergen, Norway), and Chan Il Park (Gyeongsang National University, Korea).

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  1. Research Unit of Genomics and Proteomics Applied to Marine and Food Industry, ANFACO-CECOPESCA, 36310, Vigo, Pontevedra, Spain

    Montserrat Espiñeira & Juan M. Vieites

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  1. Montserrat Espiñeira
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  2. Juan M. Vieites
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Correspondence to Montserrat Espiñeira.

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Espiñeira, M., Vieites, J.M. Authentication of the most important species of rockfish by means of fins. Eur Food Res Technol 235, 929–937 (2012). https://doi.org/10.1007/s00217-012-1824-6

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  • DOI: https://doi.org/10.1007/s00217-012-1824-6

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