Abstract
Geobacillus stearothermophilus is a thermophilic bacterium typically responsible for the flat-sour spoilage of low-acid canned food with high water activity. Control of vegetative cells and spores of G. stearothermophilus strains CECT 48 and CECT 49 by enterocin EJ97 produced by Enterococcus faecalis EJ97 is described. Both strains were highly sensitive to EJ97 in a culture medium. In samples from canned foods inoculated with a cocktail of vegetative cells or endospores of the two strains and stored at 45 °C for 30 days, viable cell counts were reduced below detection levels. The time course of microbial inactivation depended on the food sample and bacteriocin concentration. Dormant endospores were resistant to EJ97 short-time treatments (5 min), but endospores activated to germinate by heat became bacteriocin sensitive. The simultaneous application of enterocin EJ97 and heat treatments (90 and 95 °C) on dormant endospores had an increased antimicrobial effect that depended both on the bacteriocin concentration and the heat temperature. Results from this study strengthen the potential of enterocin EJ97 for biopreservation against G. stearothermophilus in canned vegetable foods and drinks.
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References
Iciek J, Papiewska A, Nowicki L (2000) Food Biotechnol. Progress in Biotechnology. Elsevier, Amsterdam, pp 385–391
Tejedor W, Rodrigo M, Martínez A (2001) J Food Prot 64:1631–1635
Dhiman SS, Sharma J, Battan B (2008) Enzym Microb Technol 43:262–269
Jay JM, Loessner MJ, Golden DA (2005) Modern food microbiology. Springer, New York, USA
Ayres JC, Mundt JO, Sandine WE (1980) Microbiology of foods. Freeman, San Francisco, CA
Feeherry FE, Munsey DT, Rowley DB (1987) Appl Environ Microbiol 53:365–370
Gordon RE, Haynes WC, Pang C (1973) Agriculture Handbook no 427. The genus Bacillus. US Department of Agriculture, Washington, DC
Russell AD (1991) J App Bacteriol 71:191–201
Cleveland J, Montville TJ, Nes IF, Chikindas ML (2001) Int J Food Microbiol 71:1–20
Devlieghere F, Vermeiren L, Debevere J (2004) Int Dairy J 14:273–285
Gálvez A, Abriouel H, Lucas López R, Ben Omar N (2007) Int J Food Microbiol 120:51–70
Mansour M, Millière JB (2001) Food Microbiol 18:87–94
Rajkovic A, Uyttendaele M, Courtens T, Debevere J (2005) Food Microbiol 22:189–197
Grande MJ, Lucas R, Abriouel H, Valdivia E, Ben Omar N, Maqueda M, Martínez-Bueno M, Martínez-Cañamero M, Gálvez A (2006) Int J Food Microbiol 106:185–194
Grande MJ, Lucas R, Abriouel H, Valdivia E, Ben Omar N, Maqueda M, Martínez-Bueno M, Martínez-Cañamero M, Gálvez A (2006) J App Microbiol 101:422–428
Grande MJ, Abriouel H, Lucas López R, Valdivia E, Ben Omar N, Martínez-Cañamero M, Gálvez A (2007) J Food Prot 70:2339–2345
Lucas R, Grande MJ, Abriouel H, Maqueda M, Ben Omar N, Valdivia E, Martínez-Cañamero M, Gálvez A (2006) Food Chem Toxicol 44:1774–1781
Cobo Molinos A, Abriouel H, Lucas López R, Ben Omar N, Valdivia E, Gálvez A (2008) Food Microbiol 25:762–770
Martínez Viedma P, Abriouel H, Ben Omar N, Lucas López R, Valdivia E, Gálvez A (2009) Food Microbiol 26:289–293
Gálvez A, Valdivia E, Abriouel H, Camafeita E, Méndez E, Martínez-Bueno M, Maqueda M (1998) Arch Microbiol 171:59–65
Franz CMAP, van Belkum MJ, Holzapfel WH, Abriouel H, Gálvez A (2007) FEMS Microbiol Rev 31:293–310
García MT, Ben Omar N, Lucas R, Pérez-Pulido R, Castro A, Grande MJ, Martínez-Cañamero M, Gálvez A (2003) Food Microbiol 20:533–536
García MT, Martínez Cañamero M, Lucas R, Ben Omar N, Pérez Pulido R, Gálvez A (2004) Int J Food Microbiol 90:161–170
García MT, Lucas R, Abriouel H, Ben Omar N, Pérez R, Grande MJ, Martínez-Cañamero M, Gálvez A (2004) J Appl Microbiol 97:731–737
Lucas R, García MT, Abriouel H, Ben Omar N, Grande MJ, Martínez-Cañamero M, Gálvez A (2007) J Ind Microbiol Biotechnol 34:779–785
Gálvez A, Maqueda M, Valdivia E, Quesada A, Montoya E (1986) Can J Microbiol 32:765–771
Heinemann B, Voris L, Stumbo CR (1965) Food Technol 19:592–596
Gould GW (1983) Mechanisms of resistance and dormancy. The bacterial spore. Academic Press Inc, New York, pp 173–209
Beard BM, Sheldon BW, Foegeding PM (1999) J Food Prot 62:484–491
Mazzotta AS, Montville TJ (1999) Appl Environ Microbiol 65:659–664
López M, González I, Condón S, Bernardo A (1996) Int J Food Microbiol 28:405–410
Palop A, Raso J, Pagan R, Condon S, Sala FJ (1999) Int J Food Microbiol 46:243–249
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We acknowledge the Research Programme of the University of Jaén, from which Pilar Martinez Viedma received a fellowship, and the Research Plan of the Junta de Andalucía (research group AGR230).
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Martínez Viedma, P., Abriouel, H., Ben Omar, N. et al. Effect of enterocin EJ97 against Geobacillus stearothermophilus vegetative cells and endospores in canned foods and beverages. Eur Food Res Technol 230, 513–519 (2010). https://doi.org/10.1007/s00217-009-1194-x
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DOI: https://doi.org/10.1007/s00217-009-1194-x