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Taking advantage of the flow cytometry technique for improving malolactic starters production

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Abstract

In bioprocesses, the industrial use of lactic acid bacteria as starter cultures is directly related to the effectiveness of preservation technologies which must guarantee high cell survival and functionality rates through the processing stages and after storage and rehydration. In this work, flow cytometry (FC) technique in combination with fluorescent probes allowed to monitor, almost in real-time conditions, the physiological states of cider malolactic starter cultures (Lactobacillus hilgardii) throughout preservation processes (freezing or freeze-drying) and their further progress during the first stages of culture implantation in the fermentation medium. A subpopulation of non-culturable but metabolically active cells, which could contribute to further fermentation, was determined when comparing FC results and viable counts. Metabolically active and dead cell counts determined by FC and colony forming units determined by the standard plate counting method showed that reactivation of frozen L. hilgardii cells during 24 h, using apple must supplemented with YE as cultivation and rehydration medium, resulted in the more suitable procedure for obtaining malolactic starter cultures.

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Acknowledgements

This work was financed by CICYT (project MCT–00-AGL-0597) from the Science and Technology Ministry, Spain. Mónica Herrero was the recipient of a postdoctoral fellowship from FICYT (Foundation for Scientific and Technological Research, Principado de Asturias, Spain). The authors wish to acknowledge the technical assistance of Ana Salas (Flow Cytometry Area, Scientific-Technical Services, University of Oviedo) and the valued advise of Jaume Comas-Riu (Scientific-Technical Services, University of Barcelona), and the support of the Asturian cidermaking industry “Sidra Escanciador, S.A” (Villaviciosa, Principado de Asturias, Spain).

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Authors and Affiliations

  1. Faculty of Chemistry, Department of Chemical Engineering and Environmental Technology, University of Oviedo, C/ Julián Clavería s/n, 33071, Oviedo, Spain

    Covadonga Quirós, Mónica Herrero, Luis A. García & Mario Díaz

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  1. Covadonga Quirós
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  2. Mónica Herrero
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  3. Luis A. García
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  4. Mario Díaz
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Correspondence to Mario Díaz.

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Quirós, C., Herrero, M., García, L.A. et al. Taking advantage of the flow cytometry technique for improving malolactic starters production. Eur Food Res Technol 228, 543–552 (2009). https://doi.org/10.1007/s00217-008-0961-4

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  • DOI: https://doi.org/10.1007/s00217-008-0961-4

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