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Immobilized 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole as solid phase luminophore in peroxyoxalate chemiluminescence

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Abstract

A new luminophore for application in peroxyoxalate chemiluminescence is presented. An analogue of the well-known chemiluminescence compound lophine, i.e. 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole (HCPI), has been covalently immobilized to controlled pore glass and a porous methacrylate resin. By using this reagent in a solid phase detection reactor, sensitive determinations of hydrogen peroxide have been demonstrated. In homogeneous solution HCPI emits poorly as a result of 1,l′-oxalyldiimidazole excitation, but when immobilized its efficiency is almost comparable to highly efficient luminophores such as 3-aminofluoranthene. Linearity extends in the single stream flow system over several orders of magnitude with both materials. The limit of detection was 1 nmol/l (10 fmole injected), when using the porous methacrylate support.

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Authors and Affiliations

  1. Department of Analytical Chemistry, University of Umeå, S-901 87, Umeå, Sweden

    Einar Pontén, Patrik Appelblad, Malin Stigbrand & Knut Irgum

  2. School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-Machi, 852, Nagasaki, Japan

    Kenichiro Nakashima

Authors
  1. Einar Pontén
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  2. Patrik Appelblad
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  3. Malin Stigbrand
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  4. Knut Irgum
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  5. Kenichiro Nakashima
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Pontén, E., Appelblad, P., Stigbrand, M. et al. Immobilized 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole as solid phase luminophore in peroxyoxalate chemiluminescence. Fresenius J Anal Chem 356, 84–89 (1996). https://doi.org/10.1007/s0021663560084

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  • DOI: https://doi.org/10.1007/s0021663560084

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