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An amino-rich polymer-coated magnetic nanomaterial for ultra-rapid separation of phosphorylated peptides in the serum of Parkinson’s disease patients

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Abstract

The elucidation of disease pathogenesis can be achieved by analyzing the low-abundance phosphopeptides in organisms. Herein, we developed a novel and easy-to-prepare polymer-coated nanomaterial. By improving the hydrophilicity and spatial conformation of the material, we effectively enhanced the adsorption of phosphopeptides and demonstrated excellent enrichment properties. The material was able to successfully enrich the phosphopeptides in only 1 min. Meanwhile, the material has high selectivity (1:2000), good loading capacity (100 μg/mg), excellent sensitivity (0.5 fmol), and great acid and alkali resistance. In addition, the material was applied to real samples, and 70 phosphopeptides were enriched from the serum of Parkinson’s disease (PD) patients and 67 phosphopeptides were enriched from the serum of normal controls. Sequences Logo showed that PD is probably associated with threonine, glutamate, serine, and glutamine. Finally, gene ontology (GO) analysis was performed on phosphopeptides enriched in PD patients’ serum. The results showed that PD patients expressed abnormal expression of the cholesterol metabolic process and cell–matrix adhesion in the biological process (BP), endoplasmic reticulum and lipoprotein in the cellular component (CC), and heparin-binding, lipid-binding, and receptor-binding in the molecular function (MF) as compared with normal individuals. All the experiments indicate that the nanomaterials have great potential in proteomics studies.

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Funding

This research is financed by the National Natural Science Foundation of China (21927805), Natural Science Foundation of Zhejiang Province (LY22B050008).

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Authors and Affiliations

  1. Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China

    Xiaoya Zhang, Bing Wang, Yiting Luo, Chuan-Fan Ding & Yinghua Yan

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  1. Xiaoya Zhang
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  2. Bing Wang
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  3. Yiting Luo
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Contributions

Xiaoya Zhang: investigation; data curation; formal analysis; validation; writing—original draft. Bing Wang: data curation; formal analysis; validation; supervision; writing—review and editing. Yiting Luo: writing—review and editing. Chuan-Fan Ding: resources, supervision. Yinghua Yan: conceptualization; methodology; writing—review and editing; resources; validation; project administration.

Corresponding authors

Correspondence to Chuan-Fan Ding or Yinghua Yan.

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The ethically approved human serums used in this study were collected with the consent of the volunteers. This study was approved by the experimental ethics committee of Ningbo University and its affiliated hospital (KS20227002).

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Zhang, X., Wang, B., Luo, Y. et al. An amino-rich polymer-coated magnetic nanomaterial for ultra-rapid separation of phosphorylated peptides in the serum of Parkinson’s disease patients. Anal Bioanal Chem (2024). https://doi.org/10.1007/s00216-024-05287-9

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