Abstract
Enzyme activity assays play a crucial role in numerous fields, including biotechnology, the food industry, and clinical diagnostics. Lipases are particularly important enzymes due to their widespread use in lipid metabolism and esterification reactions. Here, we present a pioneering method for the sensitive and selective determination of lipase activity using green carbon dots (G-CDs) for first time. G-CDs are a fascinating class of carbon nanomaterials with unique optical properties and biocompatibility, making them ideal candidates for enzyme activity assays. This approach eliminates the need for traditional fluorophores or chromogenic substrates, reducing costs, fast response time (1 min), and environmental impact with a quantum yield (QY) of 7.42%. As designed, the G-CDs fluorescent probe turn-on demonstrated a reliable linear detection range from 0 to 9 mg/mL under ideal conditions, with detection limit of 0.01 mg/mL and limit of quantification (LOQ) of 0.045 mg/mL, respectively. Furthermore, the G-CDs system was thoroughly evaluated in human serum samples, showing recoveries ranging from 100.0 to 105.0%. These findings highlight the promising applicability of the G-CDs probe for lipase detection, yielding highly favorable results.
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Funding
This work was supported by the Light of West China Program from Chinese Academy of Science (xbzg-zdsys-202008) and Regional Collaborative Innovation Project of Xinjiang (2020E01051), ANSO Scholarship for Young Talents in China, for the Chinese Academy of Sciences, and “Sponsored by UCAS Scholarship for International Students.
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Haitham Saad Al-mashriqi: visualization, methodology, investigation, data curation, validation, writing—original draft. Pascaline Sanga: software and methodology. Jia Chen: investigation, supervision, writing and revision. Xin Li: methodology. Jing Xiao: methodology. Yan Li: methodology discussion, writing and revision. Hongdeng Qiu: supervision and writing—review and editing.
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Experimental procedure on human serum was undertaken following the National Guidelines and was approved by the ethics committee of Gansu Provincial Hospital and Lanzhou Institute of Chemical Physics (Lanzhou, China).
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Al-mashriqi, H.S., Sanga, P., Chen, J. et al. Green-emitting carbon dots as a “turn on” fluorescence bio-probe for highly sensitive and selective detection of lipase in human serum. Anal Bioanal Chem 416, 971–981 (2024). https://doi.org/10.1007/s00216-023-05086-8
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DOI: https://doi.org/10.1007/s00216-023-05086-8