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A point-of-care chemiluminescence immunoassay for pepsinogen I enables large-scale community health screening

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A Correction to this article was published on 09 July 2021

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Abstract

Pepsinogen I (PGI) can reflect the morphology and function of the gastric mucosa. Accordingly, the large-scale community health screening of PGI can dramatically increase the early diagnosis rate of gastric cancer. However, PGI testing can only be carried out in comprehensive hospitals and health examination centers. To ameliorate this issue, a point-of-care chemiluminescent immunoassay for PGI was developed in a fully automated miniaturized instrument. This instrument was especially developed for health check-ups in the grassroots communities; its volume of which is only 0.18 m3. Critically, the entire detection process for a single sample only requires 20 min, and the samples can be loaded continuously, making the method suitable for high-throughput analysis. The assay displayed an excellent detection limit of 0.048 ng/mL with a broad detection range of 0–200 ng/mL. Furthermore, this assay exhibited high sensitivity and specificity, had low intra- and inter-assay coefficients of variation (<10%), and was not affected after storage at 37 °C for 7 days. The assay was used to detect PGI in 95 clinical serum samples, and the results were highly correlated with those that were clinically tested (correlation coefficient, R2 = 0.998). Hence, the method established in this work has great application value and can be broadly applied for the large-scale screening of gastric cancer in resource-limited areas.

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Funding

This work was supported by the Science and Technology Program of Guangdong Province (2021B1212050016), the Postdoctoral Scientific Research Project of Guangzhou (BHSKY20200301), the R & D Project in key areas of Guangdong Province (2020B1111160004), and Guangzhou Science and Technology Program (201902010003, 201802010030).

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Author notes

  1. Fangfang Liu and Jingjing Zou contributed equally to this work.

Authors and Affiliations

  1. College of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China

    Fangfang Liu, Jingjing Zou, Xiangxiang Luo, Yu Liu & Xiaowei He

  2. Department of Laboratory Medicine, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou, 510180, China

    Yu Wang

  3. National & Local United Engineering Lab of Rapid Diagnostic Test, Guangzhou Wondfo Biotech Co., Ltd., Guangzhou, 5l0663, China

    Chunrong Huang

Authors
  1. Fangfang Liu
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  4. Yu Liu
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Corresponding authors

Correspondence to Xiaowei He or Yu Wang.

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The clinical serum and plasma samples were approved by the Research Ethics Committee of Third Affiliated Hospital of Guangzhou University of Chinese Medicine (AF/SC-08/02.0), and the study was performed in accordance with the relevant guidelines.

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"The original online version of this article was revised: The last name of the coauthor (Jingjing Zhou) has been wrongly written, which should be ‘Zou’ instead of ‘Zhou’." plus the same explanatory text of the problem as in the erratum/correction article

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Liu, F., Zou, J., Luo, X. et al. A point-of-care chemiluminescence immunoassay for pepsinogen I enables large-scale community health screening. Anal Bioanal Chem 413, 4493–4500 (2021). https://doi.org/10.1007/s00216-021-03412-6

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  • DOI: https://doi.org/10.1007/s00216-021-03412-6

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