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Directly transferring pepper constituents to triangular papers for pungency determination by paper spray ionization mass spectrometry

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Abstract

A method named imprint paper spray ionization mass spectrometry (imprint-PSI-MS) has been developed and employed for the determination of pungency of peppers. A pepper fruit was cut into a triangular shape, deposited onto a triangular paper, and compressed by a homemade press tool aiming to imprint and transfer the pepper constituents onto the paper surface. Subsequently, the triangular paper was submitted to conventional PSI-MS analysis. Twelve peppers were analyzed, ranging from highly pungent to lowly pungent taste. Pepper pungency values from the Scoville scale (in Scoville heat units, SHU) were compared with the ion intensities of the capsaicin and dihydrocapsaicin compounds obtained from the imprint-PSI-MS analysis, and a correlation coefficient of 0.97 was achieved. In addition, the ion intensities of a sugar compound were monitored in all peppers, and the results were compared with the Scoville scale. Low sugar ion intensities were detected in pungent peppers, while high ion intensities were achieved in low-pungent peppers, suggesting that the pepper pungency may be determined by inversely relating pungency to sugar contents. This work demonstrates the utility of the imprint-PSI-MS method to perform rapid qualitative analyses of peppers and estimate the pungency by monitoring the pepper metabolites.

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Acknowledgments

The authors thank CAPES and SEDUC-Goiás for institutional support.

Funding

This study received financial support from CAPES and SEDUC-Goiás.

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Authors and Affiliations

  1. Chemistry Institute, Federal University of Goiás, Goiânia, GO, 74690-900, Brazil

    Ruver R. F. Ramalho, Lidya C. da Silva, Lanaia I. L. Maciel, Igor Pereira, Rosineide C. Simas & Boniek G. Vaz

  2. School of Agronomy, Federal University of Goiás, Goiânia, GO, 74690-900, Brazil

    Abadia dos R. Nascimento

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  1. Ruver R. F. Ramalho
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  2. Lidya C. da Silva
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  3. Lanaia I. L. Maciel
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  5. Abadia dos R. Nascimento
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Correspondence to Boniek G. Vaz.

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Ramalho, R.R.F., da Silva, L.C., Maciel, L.I.L. et al. Directly transferring pepper constituents to triangular papers for pungency determination by paper spray ionization mass spectrometry. Anal Bioanal Chem 412, 5389–5396 (2020). https://doi.org/10.1007/s00216-020-02755-w

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