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Fast screening method for molecular recognition of islet amyloid polypeptide from whole blood samples collected from diabetic patients with disposable stochastic sensors obtained by nanolayer, and nanolayer by nanolayer deposition using cold plasma

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Abstract

Nanolayer and nanolayer by nanolayer deposition of nanofilms of Ag and C using cold plasma in sequences (Ag, Ag-C, Ag-Ag-C), on porous paper, were used to design three disposable stochastic sensors for the assay of amyloid polypeptide from whole blood. The nanofilms were modified with α-cyclodextrin. The test developed using the nanofilm-based disposable stochastic sensors is used for early detection of diabetes. The wider linear concentration range (1.00 × 10−6–1.00 ng mL−1) and the lower limit of quantification (1.00 × 10−6ng mL−1) were obtained using the disposable stochastic sensors based on Ag-C and Ag-Ag-C, while the highest sensitivity (3.19 × 104 s−1/μg mL−1) was recorded using the disposable stochastic sensor based on Ag-Ag-C. The screening methods were fully validated using whole blood samples from confirmed patients, when the recovery of the islet amyloid polypeptide was higher than 98.00%.

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Funding

This work received financial support from UEFISCDI, PNCDI III framework, PN-III-P4-ID-PCE-2016-0120, and the project LAPLAS VI number PN 19150101/2020.

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Authors and Affiliations

  1. Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independenţei Str., 060021, Bucharest-6, Romania

    Raluca-Ioana Stefan-van Staden & Ioana Popa-Tudor

  2. National Institute for Laser, Plasma & Radiation Physics, 409 Str Atomistilor, 077125, Măgurele, Romania

    Marius Badulescu & Alexandru Anghel

Authors
  1. Raluca-Ioana Stefan-van Staden
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  2. Ioana Popa-Tudor
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  3. Marius Badulescu
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  4. Alexandru Anghel
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Corresponding authors

Correspondence to Raluca-Ioana Stefan-van Staden or Marius Badulescu.

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The authors declare that they have no conflicts of interest.

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Informed consent was obtained from all patients.

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Dedicated to Professor Hassan Y. Aboul-Enein on his 75th Birthday.

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Stefan-van Staden, RI., Popa-Tudor, I., Badulescu, M. et al. Fast screening method for molecular recognition of islet amyloid polypeptide from whole blood samples collected from diabetic patients with disposable stochastic sensors obtained by nanolayer, and nanolayer by nanolayer deposition using cold plasma. Anal Bioanal Chem 412, 4135–4141 (2020). https://doi.org/10.1007/s00216-020-02646-0

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  • DOI: https://doi.org/10.1007/s00216-020-02646-0

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