Abstract
Protein glycosylation plays a key role in many biological processes. In this study, a novel carbon material with nanopores was prepared by carbonization of metal–organic framework (MOF) Mil-101(Cr). The parent MOF assembled from metal ions with bridging organic linkers had many fascinating properties, such as ultrahigh surface area, suitable nanopore structure, and especially a large amount of carbon after being calcined. Due to the strong interactions between carbon and glycans as well as the size-exclusion effect of pore against protein, the N-linked glycans from standard glycoprotein or complex human serum proteins could be identified with high efficiency. The simple synthesis method as well as good enrichment efficiency made this novel carbon material a promising tool for glycosylation research.
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Acknowledgments
This work was supported by National Basic Research Program of China (Project: 2012CB910604), the National High-Tech R&D Program (Project: 2012AA020202), and the National Natural Science Foundation of China (Project: 21275034 and 21475027).
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The research followed the tenets of the Declaration of Helsinki, and the use of the human serum samples for research was approved by the Ethics Committee of Zhongshan Hospital, Fudan University. All individual participants gave informed consent for the use of these samples.
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Published in the topical collection Glycomics, Glycoproteomics and Allied Topics with guest editors Yehia Mechref and David Muddiman.
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Wang, Y., Wang, J., Gao, M. et al. A novel carbon material with nanopores prepared using a metal–organic framework as precursor for highly selective enrichment of N-linked glycans. Anal Bioanal Chem 409, 431–438 (2017). https://doi.org/10.1007/s00216-016-9796-1
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DOI: https://doi.org/10.1007/s00216-016-9796-1