Skip to main content
SpringerLink
Log in

Recent trends in the analysis of bioactive peptides in milk and dairy products

  • Trends
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Food-derived constituents represent important sources of several classes of bioactive compounds. Among them peptides have gained great attention in the last two decades thanks to the scientific evidence of their beneficial effects on health in addition to their established nutritional value. Several functionalities for bioactive peptides have been described, including antioxidative, antihypertensive, anti-inflammatory, immunomodulatory, and antimicrobial activity. They are now considered as novel and potential dietary ingredients to promote human health, though in some cases they may also have detrimental effects on health. Bioactive peptides can be naturally occurring, produced in vitro by enzymatic hydrolysis, and formed in vivo during gastrointestinal digestion of proteins. Thus, the need to gain a better understanding of the positive health effects of food peptides has prompted the development of analytical strategies for their isolation, separation, and identification in complex food matrices. Dairy products and milk are potential sources of bioactive peptides: several of them possess extra-nutritional physiological functions that qualify them to be classified under the functional food label. In this trends article we briefly describe the state-of-the-art of peptidomics methods for the identification and discovery of bioactive peptides, also considering recent progress in their analysis and highlighting the difficulty in the analysis of short amino acid sequences and endogenous peptides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Carrasco-Castilla J, Hernández-Álvarez AJ, Jiménez-Martínez C, Gutiérrez-López GF, Dávila-Ortiz G. Use of proteomics and peptidomics methods in food bioactive peptide science and engineering. Food Eng Rev. 2012;4:224–43.

  2. Sauer S, Luge T. Nutriproteomics: Facts, concepts, and perspectives. Proteomics. 2015;15:997–1013.

  3. Schrader M, Schulz-Knappe P, Fricker LD. Historical perspective of peptidomics. EuPA Open Proteome. 2014;3:171–82.

  4. Sànchez-Rivera L, Martìnez-Maqueda D, Cruz-Huerta E, Miralles B, Recio I. Peptidomics for discovery, bioavailability and monitoring of dairy bioactive peptides. Food Res Int. 2014;63:170–81.

  5. Panchaud A, Affolter M, Kussmann M. Mass spectrometry for nutritional peptidomics: how to analyze food bioactives and their health effects. J Proteome. 2012;75:3546–59.

  6. Schanbacher FL, Talhouk RS, Murray FA. Biology and origin of bioactive peptides in milk. Livest Prod Sci. 1997;50:105–23.

  7. Atanasova J, Ivanova I. Antibacterial peptides from goat and sheep milk proteins. Biotechnol Biotechnol Equip. 2010;24:1799–803.

  8. Zambrowicz A, Timmer M, Polanowski A, Lubec G, Trziszka T. Manufacturing of peptides exhibiting biological activity. Amino Acids. 2013;44:315–20.

  9. Pihlanto A. Antioxidative peptides derived from milk proteins. Int Dairy J. 2006;16:1306–14.

  10. Benkerroum N. Antimicrobial peptides generated from milk proteins: a survey and prospects for application in the food industry. A review. Int J Dairy Technol. 2010;63:320–38.

  11. Bidasolo IB, Ramos M, Gomez-Ruiz JA. In vitro simulated gastrointestinal digestion of donkeys’ milk. Peptide characterization by high performance liquid chromatography-tandem mass spectrometry. Int Dairy J. 2012;24:146–52.

  12. Li-Chan ECY. Bioactive peptides and protein hydrolysates: research trends and challenges for application as nutraceuticals and functional food ingredients. Curr Opin Food Sci. 2015;1:28–37.

  13. Udenigwe CC. Bioinformatics approaches, prospects and challenges of food bioactive peptide research. Trends Food Sci Technol. 2014;36:137–43.

  14. Saavedra L, Hebert EM, Minahk C, Ferranti P. An overview of “omic” analytical methods applied in bioactive peptide studies. (2013) 54:925–934

  15. Holton TA, Vijayakumar V, Khaldi N. Bioinformatics: current perspectives and future directions for food and nutritional research facilitated by a Food-Wiki database. Trends Food Sci Technol. 2013;34:5–17.

  16. Udenigwe CC, Gong M, Wu S. In silico analysis of the large and small subunits of cereal RuBisCO as precursors of cryptic bioactive peptides. Process Biochem. 2013;48:1794–9.

  17. Lacroix IM, Li-Chan EC. Evaluation of the potential of dietary proteins as precursors of dipeptidyl peptidase (DPP)-IV inhibitors by an in silico approach. J Funct Foods. 2012;4:403–22.

  18. Minkiewicz P, Dziuba J, Iwaniak A, Dziuba M, Darewicz M. BIOPEP database and other programs for processing bioactive peptide sequences. J AOAC Int. 2008;91:965–80.

  19. Théolier J, Fliss I, Jean J, Hammami R. MilkAMP: a comprehensive database of antimicrobial peptides of dairy origin. Dairy Sci Technol. 2014;94:181–93.

  20. Pisanu S, Pagnozzi D, Pes M, Pirisi A, Roggio T, Uzzau S, et al. Differences in the peptide profile of raw and pasteurised ovine milk cheese and implications for its bioactive potential. Int Dairy J. 2015;42:26–33.

  21. Guinane CM, Kent RM, Norberg S, O’Connor PM, Paul D, Cotter PD, et al. Generation of the antimicrobial peptide caseicin A from casein by hydrolysis with thermolysin enzymes. Int Dairy J. 2015;49:1–7.

  22. Guerrero A, Dallas DC, Contreras S, Chee S, Parker EA, Sun X, et al. Mechanistic peptidomics: factors that dictate the specificity on the formation of endogenous peptides in human milk. Mol Cell Proteomics. 2014;13:3343–51.

  23. Espejo-Carpio FJ, Pérez-Gálvez R, Almécija Mdel C, Guadix A, Guadix EM. Production of goat milk protein hydrolysate enriched in ACE-inhibitory peptides by ultrafiltration. J Dairy Res. 2014;81:385–93.

  24. Powera O, Fernándezb A, Norrisa R, Rierab FA, FitzGerald RJ. Selective enrichment of bioactive properties during ultrafiltration of a tryptic digest of β-lactoglobulin. J Funct Foods. 2014;9:38–47.

  25. Finoulst I, Pinkse M, Van Dongen W, Verhaert P. Sample preparation techniques for the untargeted LC-MS-based discovery of peptides in complex biological matrices. J Biomed Biotechnol. 2011;2011:1–14.

  26. Capriotti AL, Cavaliere C, Foglia P, Piovesana S, Samperi R, Zenezini Chiozzi R, et al. Development of an analytical strategy for the identification of potential bioactive peptides generated by in vitro tryptic digestion of fish muscle proteins. Anal Bioanal Chem. 2014;407:845–54.

  27. Català-Clariana S, Benavente F, Giménez E, Barbosa J, Sanz-Nebot V. Identification of bioactive peptides in hypoallergenic infant milk formulas by capillary electrophoresis-mass spectrometry. Anal Chim Acta. 2010;683:119–25.

  28. Herrero M, Ibañez E, Cifuentes A. Capillary electrophoresis-electrospray-mass spectrometry in peptide analysis and peptidomics. Electrophoresis. 2008;29:2148–60.

  29. Fournier ML, Gilmore JM, Martin-Brown SA, Washburn MP. Multidimensional separations-based shotgun proteomics. Chem Rev. 2007;107:3654–86.

  30. Sommella E, Pepe G, Ventre G, Pagano F, Manfra M, Pierri G, et al. Evaluation of two sub-2μm stationary phases, core-shell and totally porous monodisperse, in the second dimension of on-line comprehensive two dimensional liquid chromatography, a case study: separation of milk peptides after expiration date. J Chromatogr. 2015;A1375:54–61.

  31. Lahrichi SL, Affolter M, Zolezzi IS, Panchaud A. Food Peptidomics: large scale analysis of small bioactive peptides - A pilot study. J Proteome. 2013;88:83–91.

  32. O’Keeffe MB, FitzGerald RJ. Identification of short peptide sequences in complex milk protein hydrolysates. Food Chem. 2015;184:140–6.

  33. Kütt ML, Malbe M, Stagsted J. Nanostructure-assisted laser desorption/ionization (NALDI) for analysis of peptides in milk and colostrum. Agron Res. 2011;9:415–20.

  34. Le Maux S, Nongonierma AB, FitzGerald RJ. Improved short peptide identification using HILIC-MS/MS: retention time prediction model based on the impact of amino acid position in the peptide sequence. Food Chem. 2015;173:847–54.

  35. Baum F, Fedorova M, Ebner J, Hoffmann R, Pischetsrieder M. Analysis of the endogenous peptide profile of milk: identification of 248 mainly casein-derived peptides. J Proteome Res. 2013;12:5447–62.

  36. Piovesana S, Capriotti AL, Cavaliere C, La Barbera G, Samperi R, Zenezini Chiozzi R, et al. Peptidome characterization and bioactivity analysis of donkey milk. J Proteome. 2015;119:21–9.

  37. Samperi R, Capriotti AL, Cavaliere C, Colapicchioni V, Zenezini Chiozzi R, Laganà. In: Picό J, editor. Food proteins and peptides. Elsevier: Amsterdam; 2015.

    Chapter  Google Scholar 

  38. Dziuba B, Dziuba M. Milk proteins-derived bioactive peptides in dairy products: molecular, biological and methodological aspects. Acta Sci Pol Technol Aliment. 2014;13:5–25.

  39. Danquah MK, Agyei D. Pharmaceutical applications of bioactive peptides. Med Biotechnol. 2012;1:5–11.

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185, Rome, Italy

    Anna Laura Capriotti, Chiara Cavaliere, Susy Piovesana, Roberto Samperi & Aldo Laganà

Authors
  1. Anna Laura Capriotti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chiara Cavaliere
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Susy Piovesana
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Roberto Samperi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Aldo Laganà
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anna Laura Capriotti.

Ethics declarations

Conflict of interest

The authors declare that there are no conflicts of interest.

Additional information

Published in the topical collection featuring Young Investigators in Analytical and Bioanalytical Science with guest editors S. Daunert, A. Baeumner, S. Deo, J. Ruiz Encinar, and L. Zhang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Capriotti, A.L., Cavaliere, C., Piovesana, S. et al. Recent trends in the analysis of bioactive peptides in milk and dairy products. Anal Bioanal Chem 408, 2677–2685 (2016). https://doi.org/10.1007/s00216-016-9303-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-016-9303-8

Keywords

Search

Navigation