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Novel workflow for combining Raman spectroscopy and MALDI-MSI for tissue based studies

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Abstract

Molecular heterogeneity of cancer is a major obstacle in tumor diagnosis and treatment. To deal with this heterogeneity, a multidisciplinary combination of different analysis techniques is of urgent need because a combination enables the creation of a multimodal image of a tumor. Here, we develop a computational workflow in order to combine matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) imaging and Raman microspectroscopic imaging for tissue based studies. The computational workflow can be used to confirm a spectral histopathology (SHP) based on one technique with another technique. In this contribution, we confirmed a Raman spectroscopic based SHP with MALDI-imaging. Owing to this combination, we could demonstrate, for a larynx carcinoma sample, that tissue types and different metabolic states could be extracted from the Raman spectra. Further investigations with the help of MALDI spectra yield a better characterization of variable epithelial differentiation and a better understanding of ongoing dysplastic alterations.

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Acknowledgments

Financial support of the Carl-Zeiss Stiftung and the German Research Foundation (DFG) for the research projects PO 563/13-1, PO 563/16-1, EG 102/5-1, and SCHU 1229/15-1 is gratefully acknowledged. F.v.E., U.S., and O.G.-L. thank the German Research Foundation (DFG) for the grant EG 102/4-1 (UltrafleXtreme) and Bruker Daltonics for the cooperation.

Conflict of interest

The authors declare that no conflict of interest exists.

Author information

Authors and Affiliations

  1. Institute of Physical Chemistry and Abbe Center of Photonics, University of Jena, Helmholtzweg 4, 07743, Jena, Germany

    Thomas Bocklitz, Katharina Bräutigam, Ferdinand von Eggeling, Michael Schmitt & Jürgen Popp

  2. Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstraße 10, 07743, Jena, Germany

    Annett Urbanek & Ulrich Schubert

  3. Department of Otorhinolaryngology, Jena University Hospital, Lessingstraße 2, 07743, Jena, Germany

    Franziska Hoffmann, Ferdinand von Eggeling, Günther Ernst & Orlando Guntinas-Lichius

  4. Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743, Jena, Germany

    Ferdinand von Eggeling & Ulrich Schubert

  5. Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745, Jena, Germany

    Ferdinand von Eggeling & Jürgen Popp

Authors
  1. Thomas Bocklitz
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  2. Katharina Bräutigam
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  3. Annett Urbanek
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  4. Franziska Hoffmann
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  5. Ferdinand von Eggeling
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  6. Günther Ernst
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  7. Michael Schmitt
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  8. Ulrich Schubert
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  9. Orlando Guntinas-Lichius
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  10. Jürgen Popp
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Corresponding authors

Correspondence to Thomas Bocklitz or Jürgen Popp.

Additional information

Thomas Bocklitz and Katharina Bräutigam contributed equally to this work and share main authorship.

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Bocklitz, T., Bräutigam, K., Urbanek, A. et al. Novel workflow for combining Raman spectroscopy and MALDI-MSI for tissue based studies. Anal Bioanal Chem 407, 7865–7873 (2015). https://doi.org/10.1007/s00216-015-8987-5

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  • DOI: https://doi.org/10.1007/s00216-015-8987-5

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