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Synthesis of [77Se]-methylselenocysteine when preparing sauerkraut in the presence of [77Se]-selenite. Metabolic transformation of [77Se]-methylselenocysteine in Wistar rats determined by LC–IDA–ICP–MS

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Abstract

The use of enriched Se isotopes as tracers has provided important information on Se metabolism. However, selenium isotopes are expensive and difficult to obtain. A simple and cheap strategy based on the production of [77Se]-methylselenocysteine ([77Se]-MeSeCys) when preparing sauerkraut in the presence of [77Se]-selenite was developed. The resulting [77Se]-MeSeCys was used for evaluating the metabolic transformation of MeSeCys in Wistar rats, by feeding them with an AIN-93 M diet containing 20 % sauerkraut enriched in [77Se]-MeSeCys. Organs (liver, kidney, brain, testicles, and heart) were obtained after seven days of treatment and subjected to total selenium and selenium-speciation analysis by high-performance liquid chromatography coupled with isotope-dilution-analysis inductively-coupled-plasma mass spectrometry (HPLC–IDA–ICP–MS). Analysis of 77Se-labeled organs revealed a prominent increase (more than 100 % Se-level enhancement) of selenium in the kidney and heart, whereas in the liver selenium concentration only increased by up to 20 % and it remained constant in the brain and testicles. 77Se-enriched-sauerkraut supplementation does not alter the concentration of other essential elements in comparison to controls except for in the heart and kidney, in which selenium was positively correlated with Mg, Zn, Cu, and Mo. HPLC–ICP–MS analysis of hydrolyzed extracts after carbamidomethylation of the 77Se-labeled organs revealed the presence of [77Se]-SeCys and an unknown Se-containing peak, the identity of which could not be verified by electrospray-ionization (ESI)-MS–MS. Low amounts of [77Se]-MeSeCys were found in 77Se-labeled liver and kidney extracts, suggesting the incorporation of this selenium species in its intact form.

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Acknowledgments

The Complutense University Group of Trace and Speciation Analysis thanks the Spanish Commission of Science and Technology (CTQ2011-22732), the Community of Madrid, Spain, and the European Community for funding the FEDER programme (S2010/AGR-1464, ANALYSIC II). María Sánchez-Martínez would also like to thank the Spanish Government for a doctoral fellowship (CTQ2008-05925). This research was also funded by project AGL2007-62044 from the Spanish Ministry of Education and Science.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Av. Complutense s/n, 28040, Madrid, Spain

    María Sánchez-Martínez, Teresa Pérez-Corona, Carmen Cámara & Yolanda Madrid

  2. Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), Juan de la Cierva 3, 28006, Madrid, Spain

    Cristina Martínez-Villaluenga, Juana Frías & Elena Peñas

  3. Department of Physiology, School of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, 18071, Granada, Spain

    Jesús M. Porres & Gloria Urbano

Authors
  1. María Sánchez-Martínez
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  2. Teresa Pérez-Corona
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  3. Cristina Martínez-Villaluenga
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  4. Juana Frías
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  5. Elena Peñas
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  7. Gloria Urbano
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  8. Carmen Cámara
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  9. Yolanda Madrid
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Correspondence to Yolanda Madrid.

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Sánchez-Martínez, M., Pérez-Corona, T., Martínez-Villaluenga, C. et al. Synthesis of [77Se]-methylselenocysteine when preparing sauerkraut in the presence of [77Se]-selenite. Metabolic transformation of [77Se]-methylselenocysteine in Wistar rats determined by LC–IDA–ICP–MS. Anal Bioanal Chem 406, 7949–7958 (2014). https://doi.org/10.1007/s00216-014-8224-7

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