Skip to main content
SpringerLink
Log in

Development of an enzyme-linked immunosorbent assay for the determination of 5-hydroxymethyl-2-furfural in food

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

5-Hydroxymethyl-2-furfural (5-HMF) is considered to be an excellent indicator of quality deterioration due to excessive heating or storage for a wide range of carbohydrate-containing foods. To facilitate its analysis, a highly selective and sensitive enzyme-linked immunosorbent assay for determination of 5-HMF in food has been developed. A specific polyclonal antibody was produced against a conjugate of 5-HMF coupled to bovine serum albumin. The IC50 and limit of method detection were 0.15 ± 0.012 mg L-1 and 0.02 ± 0.002 mg L-1, respectively. The proposed method was applied to detect 5-HMF in French mini bread, potato chips, French soft bread, and wheat chicken nuggets with recoveries ranging from 84.07 to 97.09% and relative standard deviation (n = 3) below 8.65% in all samples. The quantitative results were in good agreement with those obtained by the high-performance liquid chromatography method, which suggests that the method developed will be very useful for monitoring 5-HMF in food samples.

Schematic figure of enzyme-linked immunosorbent assay for 5-HMF

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Jobstla D, Husoy T, Alexander J, Bjellaas T, Leitner E, Murkovic M (2010) Food Chem 123:814–818

    Article  Google Scholar 

  2. Bachmann S, Meier M, Kanzig A (1997) Lebensmittelchemie 51:49–50

    Google Scholar 

  3. Ramirez-Jimenez A, Guerra-Hernandez E, Gracia-Villanova B (2000) J Agric Food Chem 48:4176–4181

    Article  CAS  Google Scholar 

  4. Severin I, Dumont C, Jondeau-Cabaton A, Graillot V, Chagnon MC (2010) Toxicol Lett 192:189–194

    Article  CAS  Google Scholar 

  5. Janzowski C, Glaab V, Samimi E, Schlatter J, Eisenbrand G (2000) Food Chem Toxicol 38:801–809

    Article  CAS  Google Scholar 

  6. Spano N, Ciulu M, Floris I, Panzanelli A, Pilo MI, Piu PC, Salis S, Sanna G (2009) Talanta 78:310–314

    Article  CAS  Google Scholar 

  7. Ibarz A, Pagan J, Garza S (2000) J Sci Food Agric 80:1162–1168

    Article  CAS  Google Scholar 

  8. Rada-Mendoza M, Luz Sanz M, Olano A, Villamiel M (2004) Food Chem 85:605–609

    Article  CAS  Google Scholar 

  9. Ameur LA, Trystram G, Birlouez-Aragon I (2006) Food Chem 98:790–796

    Article  CAS  Google Scholar 

  10. Ramirez-Jimenez A, Garcia-Villanova B, Guerra-Hernandez E (2001) J Sci Food Agric 81:513–518

    Article  CAS  Google Scholar 

  11. Ramirez-Jimenez A, Garcia-Villanova B, Guerra-Hernandez E (2000) Food Res Int 33:833–838

    Article  CAS  Google Scholar 

  12. Ramirez-Jimenez A, Guerra-Hernandez E, Garcia-Villanova B (2000) J Agric Food Chem 48:4176–4181

    Article  CAS  Google Scholar 

  13. Ferrer E, Alegria A, Farre R, Abellan P, Romero F (2002) J Chromatogr A 947:85–95

    Article  CAS  Google Scholar 

  14. Ramirez-Jimenez A, Guerra-Hernandez E, Garcia-Villanova B (2003) Food Chem 83:219–225

    Article  CAS  Google Scholar 

  15. Garcia-Villanova B, Guerra-Hernandez E, Martinez Gomez E, Montilla J (1993) J Agric Food Chem 41:1254–1255

    Article  CAS  Google Scholar 

  16. Hryncewicz CL, Koberda M, Konkowski MS (1996) J Pharm Biomed Anal 14:429–434

    Article  CAS  Google Scholar 

  17. Martysiak-Zurowska D, Borowicz A (2009) Chem Anal 54:939–947

    CAS  Google Scholar 

  18. Zappala A, Fallico B, Arena E, Verzera A (2005) Food Control 16:273–277

    Article  CAS  Google Scholar 

  19. Chavez-Servin JL, Castellote AI, Lopez-Sabater MC (2006) Food Res Int 39:536–543

    Article  CAS  Google Scholar 

  20. Michail K, Juan H, Maier A, Matzi V, Greilberger J, Wintersteiger R (2007) Anal Chim Acta 581:287–297

    Article  CAS  Google Scholar 

  21. Gaspar EMSM, Lopes JF (2009) J Chromatogr A 1216:2762–2767

    Article  CAS  Google Scholar 

  22. Rodrigues JA, Barros AS, Carvalho B, Brandao T, Gil AM, Ferreira ACS (2011) J Chromatogr A 1218:990–996

    Article  CAS  Google Scholar 

  23. Jun M, Shao Y, Ho CT, Koetter U, Lech S (2003) J Agric Food Chem 51:6340–6346

    Article  CAS  Google Scholar 

  24. Li Zl, Wang S, Lee NA, Allan RD, Kennedy IR (2004) Anal Chim Acta 503:171–177

    Article  CAS  Google Scholar 

  25. Won CY, Chu CC (1998) Carbohydr Polym 36:327–334

    Article  CAS  Google Scholar 

  26. Zhang Y, Guo TX, Yu J, Feng F, Wang S (2010) Chin J Food Sci 31:212–215

    Google Scholar 

  27. Szurdoki F, Kido H, Hammock BD (1995) Bioconjug Chem 6:145–149

    Article  CAS  Google Scholar 

  28. Bao HJ, Fang S, Liu ZJ, Shi HY, Ye YH, Wang MH (2010) J Agric Food Chem 58:8167–8170

    Article  CAS  Google Scholar 

  29. Ferrer E, Alegria A, Farre R, Abellan P, Romero F (2005) Food Chem 89:639–645

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors are grateful for financial support from the Ministry of Science and Technology of China (project nos. 2009BADB9B07 and 2009BADB9B06) and the National Natural Science Foundation of China (31171683).

Author information

Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China

    Guozhen Fang, Yanyan Lv, Wei Sheng, Bing Liu, Xiaoxue Wang & Shuo Wang

Authors
  1. Guozhen Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yanyan Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wei Sheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bing Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaoxue Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shuo Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shuo Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fang, G., Lv, Y., Sheng, W. et al. Development of an enzyme-linked immunosorbent assay for the determination of 5-hydroxymethyl-2-furfural in food. Anal Bioanal Chem 401, 3367–3373 (2011). https://doi.org/10.1007/s00216-011-5430-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-011-5430-4

Keywords

Search

Navigation