Abstract
Methodology for detection of activated benzo[a]pyrene (B[a]P)–nucleoside adducts by liquid chromatography–tandem mass spectrometry is reported. Adducts of B[a]P-dihydrodiol epoxide (B[a]PDE) with guanosine and adenosine have been detected for the first time by use of precursor ion scan and neutral loss scan. B[a]P was then activated by use of UV irradiation and some of the products obtained have been identified by taking advantage of the information obtained for B[a]PDE. Photoactivation has also been carried out in the presence of hydrogen peroxide; this resulted in a higher yield of products with increased production of BaP diones. The reactivity of these compounds toward nucleosides has been tested. The proposed method was successfully used for detection of one stable guanosine–B[a]P dione adduct.
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Baird WM, Hooven LA, Mahadevan B (2005) Environ Mol Mutagen 45:106–114
Boffetta P, Jourenkova N, Gustavsson P (1997) Cancer Causes Control 8:444–472
Pfeifer GP, Denissenko MF, Olivier M, Tretyakova N, Hecht SS, Hainaut P (2002) Oncogene 21:7435–7451
Armstrong B, Hutchinson E, Unwin J, Fletcher T (2004) Environ Health Perspect 112:970–978
Penning TM, Burczynski ME, Hung CF, McCoull KD, Palackal NT, Tsuruda LS (1999) Chem Res Toxicol 12:1–18
Poirier MC, Beland FA (1992) Chem Res Toxicol 5:749–757
Ross JA, Nelson GB, Rabinowits JR, Stoner GD, Nesnow S, Mass M (1996) Polycycl Aromat Comp 10:267–271
Rindgen D, Turesky RJ, Vouros P (1995) Chem Res Toxicol 8:1005–1013
Wang JJ, Marshall WD, Frazer DG, Law B, Lewis DM (2003) Anal Biochem 322:79–88
Casale GP, Singhal M, Bhattacharya S, RamaNathan R, Roberts KP, Barbacci DC, Zhao J, Jankowiak R, Gross ML, Cavalieri EL, Small GJ, Rennard SI, Mumford JL, Shen M (2001) Chem Res Toxicol 14:192–201
Divi RL, Beland FA, Fu PP, Von Tungeln LS, Schoket B, Camara JE, Ghei M, Rothman N, Sinha R, Poirier MC (2002) Carcinogenesis 23:2043–2049
Wang JJ, Frazer DG, Law B, Lewis DM (2003) Analyst 128:864–870
Nestmann ER, Bryant DW, Carr CJ (1996) Regul Toxicol Pharmacol 24:9–18
Toyooka T, Ibuki Y (2007) Environ Toxicol Pharmacol 23:256–263
Yang J, Wang L, Fu PP, Yu H (2004) Mutat Res 557:99–108
Platt KL, Aderhold S, Kulpe K, Fickler M (2008) Mutat Res 650:96–103
Shemer H, Linden KG (2007) Water Res 41:853–861
Kot-Wasik A, Dabrowska D, Namiesnik J (2004) J Photochem Photobiol A 168:109–115
Reed M, Monske M, Lauer F, Meserole S, Born J, Burchiel S (2003) J Toxicol Environ Health A 66:1189–1205
Esmans E, Broes D, Hoes I, Lemière F, Vanhoutte K (1998) J Chromatogr A 794:109–127
Devanesan PD, Higginbotham S, Ariese F, Jankowiak R, Suh M, Small GJ, Cavalieri EL, Rogan EG (1996) Chem Res Toxicol 9:1113–1116
Rogan EG, Devanesan PD, Ramakrishna NV, Higginbotham S, Padmavathi NS, Chapman K, Cavalieri EL, Jeong H, Jankowiak R, Small GJ (1993) Chem Res Toxicol 6:356–363
Suzuki N, Prabhu P, Shibutani S (2004) In: Yan Z, Caldwell GW (eds) Optimization in drug discovery. New York, Springer
Del Carlo M, Di Marcello M, Perugini M, Ponzielli V, Sergi M, Mascini M, Compagnone D (2008) Microchim Acta 163:163–169
Wang LR, Qu N, Guo LH (2008) Anal Chem 80:3910–3914
Farmer PB (2004) Toxicol Lett 149:3–9
Phillips DH (1983) Nature 303:468–472
Chen YL, Wang CJ, Wu KY (2005) Rapid Commun Mass Spectrom 19:893–898
Feng F, Wang X, Yuan H, Wang H (2009) J Chromatogr B 877:2104–2112
Feng F, Yin J, Song M, Wang H (2008) J Chromatogr A 1183:119–128
Wang JJ, Marshall WD, Law B, Lewis DM (2003) Int J Mass Spectrom 230:45–55
Barry JP, Norwood C, Vouros P (1996) Anal Chem 68:1432–1438
Branco PS, Chiarelli MP, Jackson OLJ, Beland FA (1995) J Am Soc Mass Spectrom 6:248–256
Xue W, Warshawsky D (2005) Toxicol Appl Pharmacol 206:73–93
Ruan Q, Kim HY, Jiang H, Penning TM, Harvey RG, Blair IA (2006) Rapid Commun Mass Spectrom 20:1369–1380
Beland FA, Churchwell MI, Von Tungeln LS, Chen S, Fu PP, Culp SJ, Schoket B, Gyorffy E, Minarovits J, Poirier MC, Bowman ED, Weston A, Doerge DR (2005) Chem Res Toxicol 18:1306–1315
Thompson AL, Hurtubise RJ (2007) Anal Chim Acta 584:28–36
Dong S, Hwang HM, Shi X, Holloway L, Yu H (2000) Chem Res Toxicol 13:585–593
Balu N, Padgett WT, Lambert GR, Swank AE, Richard AM, Nesnow S (2004) Chem Res Toxicol 17:827–838
Balu N, Padgett WT, Nelson GB, Lambert GR, Ross JA, Nesnow S (2006) Anal Biochem 355:213–223
Lintelmann J, Fischer K, Matuschek G (2006) J Chromatogr A 1133:241–247
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Compagnone, D., Curini, R., D’Ascenzo, G. et al. Neutral loss and precursor ion scan tandem mass spectrometry for study of activated benzopyrene–DNA adducts. Anal Bioanal Chem 401, 1983–1991 (2011). https://doi.org/10.1007/s00216-011-5261-3
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DOI: https://doi.org/10.1007/s00216-011-5261-3