Abstract
The first liquid chromatography–tandem mass spectrometry method was developed and validated for the simultaneous quantification of p-aminohippuric acid and inulin, both typical biomarkers of kidney function. 5-(Hydroxymethyl)furfural, generated from inulin by acid and heat preparation, was used as an inulin substitute for the quantification. Acetaminophen was used as the internal standard. Solid-phase extraction was carried out with 5% methanol as the washing solution to optimize the retention of the analytes and to avoid obstruction of the orifice plate of the mass spectrometer caused by any unreacted inulin residue remaining from the sample preparation process. Chromatography separation was performed on a Symmetry C18 column and a mobile phase composed of 2 mM ammonium formate and 0.1% formic acid in water (solvent A) and 2 mM ammonium formate and 0.1% formic acid in acetonitrile (solvent B) (30:70, v/v). Detection was performed with a triple-quadrupole tandem mass spectrometer using positive ion mode electrospray ionization in the multiple reaction monitoring mode. The selected transitions were m/z 195.2 → 120.2, 127.1 → 109.1, and 152.1 → 110.0 for p-aminohippuric acid, inulin [measured as 5-(hydroxymethyl)furfural], and acetaminophen, respectively. The linearity ranged from 10 to 140 μg/mL and from 100 to 1,400 μg/mL for p-aminohippurric acid and inulin (r > 0.99), respectively. The precisions and accuracies were all within 12 and 11% for the lower limit of quantification and quality control samples, respectively. This application was proven to be reliable and accurate and was successfully applied to a renal function study.
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References
Baccard N, Hoizey G, Frances C, Lamiable D, Trenque T, Millart H (1999) Analyst 124:833–836
Cole BR, Giangiacomo J, Ingelfinger JR, Robson AM (1972) N Engl J Med 287:1109–1114
Pastore A, Bernardini S, Dello Strologo L, Rizzoni G, Cortese C, Federici G (2001) J Chromatogr B Biomed Sci Appl 751:187–191
Decosterd LA, Karagiannis A, Roulet JM, Belaz N, Appenzeller M, Buclin T, Vogel P, Biollaz J (1997) J Chromatogr B Biomed Sci Appl 703:25–36
Narins RG, Krishna GG, Riley LJ (1992) Assessment of renal function: characteristics of the functional and organic forms of acute renal failure. Raven, New York
Shemesh O, Golbetz H, Kriss JP, Myers BD (1985) Kidney Int 28:830–838
Rolin HA 3rd, Hall PM, Wei R (1984) Am J Kidney Dis 4:48–54
Price M (1972) J Urol 107:339–344
Masereeuw R, Moons MM, Smits P, Russel FG (1996) Br J Pharmacol 119:57–64
Mak RH, Al Dahhan J, Azzopardi D, Bosque M, Chantler C, Haycock GB (1983) Kidney Int 23:410–413
Marsilio R, Naturale M, Manghi P, Montini G, Murer L, Ros M, Bisogno G, Andretta B, Dussini N, Giordano G, Zacchello G, Dall’Amico R (2000) J Chromatogr B Biomed Sci Appl 744:241–247
Takahashi N, Boysen G, Li F, Li Y, Swenberg JA (2007) Kidney Int 71:266–271
Walser M, Davidson DG, Orloff J (1955) J Clin Invest 34:1520–1523
Heyrovsky A (1956) Clin Chim Acta 1:470–474
Fjeldbo W, Stamey TA (1968) J Lab Clin Med 72:353–358
Pearson RM (1979) Br J Clin Pharmacol 7:129–138
Boschi S, Marchesini B (1981) J Chromatogr 224:139–143
Agarwal R (1998) J Chromatogr B Biomed Sci Appl 705:3–9
Gabel RA, Ranaei RA, Kivlighn SD (1996) J Pharmacol Toxicol Methods 36:189–197
Prueksaritanont T, Chen ML, Chiou WL (1984) J Chromatogr 306:89–97
Jenny PD, Weber A, Smith AL (1989) J Chromatogr 490:213–218
Song DJ, Hsu KY (1996) J Chromatogr B Biomed Appl 677:69–75
Dowling TC, Frye RF, Zemaitis MA (1998) J Chromatogr B Biomed Sci Appl 716:305–313
Ruo TI, Wang Z, Dordal MS, Atkinson AJJ (1991) Clin Chim Acta 204:217–222
Dall’Amico R, Montini G, Pisanello L, Piovesan G, Bottaro S, Cracco AT, Zacchello G, Zacchello F (1995) J Chromatogr B Biomed Appl 672:155–159
U.S. Food and Drug Administration, Guidance for Industry, Bioanalytical methods validation (2001). Published by: U.S. Department of Health and Human Services Food and Drug Administration, Center for Drug Evaluation and Research (CDER), Center for Veterinary Medicine (CVM) May 2001 BP http://www.fda.gov/cder/guidance/index.htm
Matuszewski BK, Constanzer ML, Chavez-Eng CM (2003) Anal Chem 75:3019–3030
Fischer PA, Bogoliuk CB, Ramirez AJ, Sanchez RA, Masnatta LD (2000) Kidney Int 58:1336–1341
Schnurr E, Lahme W, Küppers H (1980) Nephrology 13:26–29
Michail K, Juan H, Maier A, Matzi V, Greilberger J, Wintersteiger R (2007) Anal Chim Acta 581:287–297
Teixido E, Moyano E, Santos FJ, Galceran MT (2008) J Chromatogr A 1185:102–108
Schneider R, Sauvant C, Betz B, Otremba M, Fischer D, Holzinger H, Wanner C, Galle J, Gekle M (2007) Am J Physiol Renal Physiol 292:F1599–F1605
Acknowledgements
This study was supported by a partial grant no. 98-70 from Cheng Hsin General Hospital. The authors are grateful to Hsien-Yuan Fan for the preparation of this manuscript.
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Lin, CC., Kuo, CW. & Pao, LH. Development and validation of a liquid chromatography–tandem mass spectrometry method for simultaneous quantification of p-aminohippuric acid and inulin in rat plasma for renal function study. Anal Bioanal Chem 398, 857–865 (2010). https://doi.org/10.1007/s00216-010-3957-4
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DOI: https://doi.org/10.1007/s00216-010-3957-4