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Determination of vancomycin in human plasma using high-performance liquid chromatography with fluorescence detection

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Abstract

A high-performance liquid chromatographic (HPLC) method with fluorescence detection for the quantification of vancomycin in human plasma was developed and validated. The method includes an extraction of vancomycin by deproteinization with acetonitrile. The analyses were carried out at 258 nm as the emission wavelength while exciting at 225 nm on a reversed-phase column (30 cm × 4 mm i.d. × 10 µm Waters Associates μBondapak C18) using a mobile phase composed of methanol and phosphate buffer at pH 6.3. Vancomycin was quantitatively recovered from human plasma samples (>96%) with high values of precision. The separation was completed within 27 min. The calibration curve was linear over the range from 5 to 1,000 ng/mL with the detection and quantification limits of 2 ng/mL and 5 ng/mL, respectively. This method is suitable for the routine assay of plasma samples.

The effect of the deproteinization solvent on the signal of the interference peak at retention time of 15.0 min. The peak which interferes with the peaks of Erythromycin and Vancomycin has been disappeared by using 2 mL acetonitrile as the deproteinization solvent.

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References

  1. Liu J, Volk KJ, Lee MS, Pucci M, Handwerger S (1994) Anal Chem 66:2412–2416

    Article  CAS  Google Scholar 

  2. Thomas G (2000) in: Medicinal Chemistry, an Introduction 155–169. Wiley, England

  3. Lin YS, Tsai PJ, Chen MF, Weng YC (2005) Anal Chem 77:1753–1760

    Article  CAS  Google Scholar 

  4. Shively ML, Thompson DC (1995) Internat J Pharm 117:119–122

    Article  CAS  Google Scholar 

  5. Al-Nassir WN, Sethi AK, Li Y, Pultz MJ, Riggs MM, Donskey CJ (2008) Antimicrob Agents Chemother 52:2403–2406

    Article  CAS  Google Scholar 

  6. Weese JS (2008) J Am Veterin Med Assoc 233:565–567

    Article  Google Scholar 

  7. Kang J, Bischoff D, Jiang Z, Bister B, Sssmuth RD, Schurig V (2004) Anal Chem 76:2387–2392

    Article  CAS  Google Scholar 

  8. Sun Q, Olesik SV (1999) Anal Chem 71:2139–2145

    Article  CAS  Google Scholar 

  9. Karlsson C, Karlsson L, Armstrong DW, Owens PK (2000) Anal Chem 72:4394–4401

    Article  CAS  Google Scholar 

  10. Armstrong DW, Tang Y, Chen S, Zhou Y, Bagwill C, Chen JR (1994) Anal Chem 66:1473–1484

    Article  CAS  Google Scholar 

  11. Slama I, Dufresne C, Jourdan E, Fahrat F, Villet A, Ravel A, Grosset C, Peyrin E (2002) Anal Chem 74:5205–5211

    Article  CAS  Google Scholar 

  12. Peter A, Torok G, Armstrong DW, Toth G, Tourwe D (1998) J Chromatogr A 828:177–190

    Article  CAS  Google Scholar 

  13. Peyrin E, Ravel A, Grosset C, Villet A, Ravelet C, Nicolle E, Alary J (2001) Chromatographia 53:645–650

    Article  CAS  Google Scholar 

  14. Peyrin E, Ravelet C, Nicolle E, Villet A, Grosset C, Ravel A, Alary J (2001) J Chromatogr A 923:37–43

    Article  CAS  Google Scholar 

  15. Slama I, Ravelet C, Grosset C, Ravel A, Villet A, Nicolle E, Peyrin E (2002) Anal Chem 74:282–287

    Article  CAS  Google Scholar 

  16. Vespalec R, Billiet H, Frank J, Bocek P (1996) Electrophoresis 17:1214–1221

    Article  CAS  Google Scholar 

  17. Armstrong DW, Rundlett K (1994) J Liq Chromatogr 17:1695–1707

    Article  CAS  Google Scholar 

  18. Strege M, Huff B, Risley DS (1996) LC-GC 14:144–150

    CAS  Google Scholar 

  19. Sharp VS, Risley DS (1999) Chirality 11:75–81

    Article  CAS  Google Scholar 

  20. Sun Q, Olesik SV (2000) J Chromatogr B 745:159–166

    Article  CAS  Google Scholar 

  21. Tang Z, Kang J (2006) Anal Chem 78:2514–2520

    Article  CAS  Google Scholar 

  22. Han J, Jiang S, Cui X, Sun Y, Li G, Zhang J, Fawcett JP, Gu J (2009) Pharm Biomed Anal 50:171–174

    Article  CAS  Google Scholar 

  23. Clarke A, Harmon B, Felippis MR (2009) Anal Biochem 390:209–211

    Article  CAS  Google Scholar 

  24. Schebb NH, Falck D, Faber H, Hein EM, Karst U, Hayen H (2009) J Chromatogr A 1216:5249–5255

    Article  CAS  Google Scholar 

  25. Vera López KJ, Bertoluci DF, Vicente KM, Dell’Aquilla AM, Jorge Santos SRC (2007) J Chromatogr B 860:241–245

    Article  Google Scholar 

  26. Farin D, Piva GA, Gozlan I, Kitzes-Cohen R (1998) J Pharm Biomed Anal 18:367–372

    Article  CAS  Google Scholar 

  27. Backes DW, Aboleneen HI, Simpson JA (1998) J Pharm Biomed Anal 16:1281–1287

    Article  CAS  Google Scholar 

  28. Mitsue S, Tomofumi S, Makoto T, Hiroshi H, Noriko U (2004) Biomed Chromatogr 18(9):735–738

    Article  Google Scholar 

  29. Favetta P, Guitton J, Bleyzac N, Dufresne C, Bureau J (2001) J Chromatogr B 751:377–382

    Article  CAS  Google Scholar 

  30. Zhang T, Watson DG, Azike C, Tettey JNA, Stearns AT, Binning AR, Payne CJ (2007) J Chromatogr B 857:352–356

    Article  CAS  Google Scholar 

  31. Ghassempour A, Darbandi MK, Asghari FS (2001) Talanta 55:573–580

    Article  CAS  Google Scholar 

  32. Shibata N, Ishida M, Prasad YVR, Gao W, Yoshikawa Y, Takada K (2003) J Chromatogr B 789:211–218

    Article  CAS  Google Scholar 

  33. Kirchmeier RL, Upton RP (1978) Anal Chem 50:349–351

    Article  CAS  Google Scholar 

  34. Li B, Zhang T, Xu Z, Fang HHP (2009) Anal Chim Acta 645:64–72

    Article  CAS  Google Scholar 

  35. Valle MJ, López FG, Navarro AS (2008) J Pharm Biomed Anal 48:835–839

    Article  Google Scholar 

  36. Ye G, Cai X, Wang B, Zhou Z, Yu X, Wang W, Zhang J, Wang Y, Dong J, Jiang Y (2008) J Pharm Biomed Anal 48:860–865

    Article  CAS  Google Scholar 

  37. Abu-Shandi KH, Redel E (2009) Jordan J Chem 4(2):183–194

    CAS  Google Scholar 

  38. Kajiro T, Nakajima Y, Fukushima T, Imai K (2002) Anal Chem 74:4519–4525

    Article  CAS  Google Scholar 

  39. Brunetto MR, Contreras Y, Clavijo S, Torres D, Delgado Y, Ovalles F, Ayala C, Gallignani M, Estela JM, Martin VC (2009) J Pharm Biomed Anal 50:194–199

    Article  CAS  Google Scholar 

  40. Gilpin RK, Gilpin CS (2007) Anal Chem 79:4275–4294

    Article  CAS  Google Scholar 

  41. http://www.fda.gov/CDER/GUIDANCE/4252fnl.pdf

  42. Snyder L, Kirkland J, Glajch J (1997) in: Practical HPLC method development, chapter 2, 21, Wiley, New York, USA

  43. Tsou T, Huang Y, Lee C, Lee A, Wang H, Chen S (2007) J Sep Sci 30:2407–2413

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge the financial support of Deutsche Forschungsgemeinschaft (DFG-Germany) and Tafila Technical University. The author would like to thank Hikma Pharmaceuticals for their help in the experiment setup and for the kind donation of vancomycin hydrochloride.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Tafila Technical University, P. O. Box 179, Tafila, 66110, Jordan

    Khalid Hamad Abu-Shandi

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  1. Khalid Hamad Abu-Shandi
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Correspondence to Khalid Hamad Abu-Shandi.

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Abu-Shandi, K.H. Determination of vancomycin in human plasma using high-performance liquid chromatography with fluorescence detection. Anal Bioanal Chem 395, 527–532 (2009). https://doi.org/10.1007/s00216-009-2948-9

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  • DOI: https://doi.org/10.1007/s00216-009-2948-9

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