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Hapten synthesis and enzyme-linked immunosorbent assay for phosmet residues: assay optimization and investigation of matrix effects from different food samples

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Abstract

In this study, a panel of haptens was synthesized for immunoconjugate preparation, and several haptens for heterologous tracer conjugates were also prepared. A highly sensitive polyclonal antibody against the organophosphorus insecticide phosmet was obtained and competitive direct enzyme-linked immunosorbent assays (cd-ELISA) for this pesticide were developed. In the cd-ELISA, the limit of detection (IC15) was 0.6 μg kg−1 and the sensitivity (IC50) was 20 μg kg−1. The suitability of the ELISA for pesticide quantification in peach, apple, orange juice, and apple juice was also studied. Good accuracy and precision were obtained with mean recoveries between 78% and 102.3% and mean coefficients of variation below 13.63%. Validation of the ELISA was conducted by high-performance liquid chromatography. The correlation between the data obtained using the microwell assay and the high-performance liquid chromatography was good (R 2 = 0.9849). The developed immunoassay methods were suitable for the rapid quantitative or qualitative determination of phosmet in food samples.

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Acknowledgements

The authors are grateful for financial supports from the Ministry of Science and Technology of the People’s Republic of China by the Chinese National “863” High-Tech Research Program (no. 2006AA10Z448).

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Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300222, China

    Yang Song, Yu Ge, Yan Zhang, Bing Liu, Yang Lu, Tingting Dong & Shuo Wang

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  1. Yang Song
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  2. Yu Ge
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  3. Yan Zhang
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  4. Bing Liu
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  5. Yang Lu
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  6. Tingting Dong
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  7. Shuo Wang
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Correspondence to Shuo Wang.

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Song, Y., Ge, Y., Zhang, Y. et al. Hapten synthesis and enzyme-linked immunosorbent assay for phosmet residues: assay optimization and investigation of matrix effects from different food samples. Anal Bioanal Chem 393, 2001–2008 (2009). https://doi.org/10.1007/s00216-009-2684-1

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  • DOI: https://doi.org/10.1007/s00216-009-2684-1

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