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Determination of triadimenol based on the quenching effect on resonance light scattering from the triadimenol–deoxyribonucleic acid–hydrochloric acid system

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Abstract

Analysis of triadimenol was carried out using deoxyribonucleic acids (DNA) via the resonance light scattering (RLS) technique. After adding triadimenol into aqueous medium of pH 1.72, the RLS of DNA was remarkably quenched. A resonance light scattering peak at 310 nm was found, and the quenched intensity of RLS at this wavelength was proportional to the concentration of triadimenol. The linear range of the calibration curve was approximately 0–3 μg mL−1 with a detection limit (S/N = 3) of 0.07 μg mL−1. The triadimenol in samples of water, cucumber and human serum was determined. The results were satisfactory, and the recovery rates were in the range of 96.3–106.0%, 94.8–105.9% and 92.3–100.5%, respectively. The interaction mechanism was also studied.

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Acknowledgements

This work was supported by Natural Science Foundations of China (No. 20575005).

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Authors and Affiliations

  1. College of Science, China Agricultural University, Beijing, 100094, China

    Fengpei Du, Xiaolin Luo, Shicong Hou, Gang Liu, Liping Ren, Li Zhang, Qin Huang & Nianqin Jie

  2. Research Center for Eco-Environmental Science, Chinese Academy of Science, Beijing, 100085, China

    Guibin Jiang

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  1. Fengpei Du
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  2. Xiaolin Luo
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  3. Guibin Jiang
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  4. Shicong Hou
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  5. Gang Liu
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  6. Liping Ren
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  7. Li Zhang
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  8. Qin Huang
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  9. Nianqin Jie
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Correspondence to Nianqin Jie.

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Du, F., Luo, X., Jiang, G. et al. Determination of triadimenol based on the quenching effect on resonance light scattering from the triadimenol–deoxyribonucleic acid–hydrochloric acid system. Anal Bioanal Chem 388, 489–493 (2007). https://doi.org/10.1007/s00216-007-1218-y

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  • DOI: https://doi.org/10.1007/s00216-007-1218-y

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