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A novel microbial biosensor based on cells of Gluconobacter oxydans for the selective determination of 1,3-propanediol in the presence of glycerol and its application to bioprocess monitoring

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Abstract

Novel and selective microbial amperometric biosensors that use Gluconobacter oxydans cells to monitor the bacterial bioconversion of glycerol (Gly) to 1,3-propanediol (1,3-PD) are described. Two different mediators, ferricyanide and flexible polyvinylimidazole osmium functionalized polymer (Os-polymer), were employed to prepare two different microbial biosensors, both of which gave high detection performance. The good operational stabilities of both types of biosensor were underlined by the ability to detect 1,3-PD throughout 140 h of continuous operation. Both microbial biosensor systems showed excellent selectivity for 1,3-PD in the presence of a high excess of glycerol [selectivity ratios (1,3-PD/Gly) of 118 or 245 for the ferricyanide and Os-polymer systems, respectively]. Further, the robustness of each microbial biosensor was highlighted by the high reliability of 1,3-PD detection achieved (average RSD of standards <2%, and well below 4% for samples). The biosensor implementing the Os-polymer mediator exhibited high selectivity towards 1,3-PD detection and allowed moderate sample throughput (up to 12 h−1) when integrated into a flow system. This system was used to monitor the concentration of 1,3-PD during a real bioprocess. Results from biosensor assays of 1,3-PD in bioprocess samples taken throughout the fermentation were in a very good agreement with results obtained from reference HPLC assays (R 2 = 0.999).

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Acknowledgements

This work was supported by the European Commission, under the scope of the BIODIOL project. The BIODIOL project (QLK5-CT-2002-01343) is funded by the European Commission within the 5th Framework Program. The integrated bioprocess development and economical evaluation of BIODIOL also includes a partner’s work, which is not presented here. The EU is not responsible for the content of this publication. This work was also supported by a grant from the Slovak Grant Agency for Science VEGA under the Projects No. 1/1196/04, 1/4299/07 and 1/4452/07.

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32, Bratislava, Slovakia

    Jaroslav Katrlík & Milan Valach

  2. Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia

    Igor Voštiar

  3. Department of GlycoBiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovakia

    Jana Šefčovičová, Ján Tkáč, Vladimír Mastihuba & Peter Gemeiner

  4. Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia

    Vladimír Štefuca

Authors
  1. Jaroslav Katrlík
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  2. Igor Voštiar
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  3. Jana Šefčovičová
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  4. Ján Tkáč
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  5. Vladimír Mastihuba
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  6. Milan Valach
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  7. Vladimír Štefuca
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  8. Peter Gemeiner
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Correspondence to Jaroslav Katrlík.

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Katrlík, J., Voštiar, I., Šefčovičová, J. et al. A novel microbial biosensor based on cells of Gluconobacter oxydans for the selective determination of 1,3-propanediol in the presence of glycerol and its application to bioprocess monitoring. Anal Bioanal Chem 388, 287–295 (2007). https://doi.org/10.1007/s00216-007-1211-5

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  • DOI: https://doi.org/10.1007/s00216-007-1211-5

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