Abstract
Simultaneous determination of the fat-soluble vitamins A and E and the water-soluble vitamins B1, B2 and B6 has been carried using a screening method from fluorescence contour graphs. These graphs show different colour zones in relation to the fluorescence intensity measured for the pair of excitation/emission wavelengths. The identification of the corresponding excitation/emission wavelength zones allows the detection of different vitamins in an aqueous medium regardless of the fat or water solubility of each vitamin, owing to the presence of a surfactant which forms micelles in water at the used concentration (over the critical micelle concentration). The micelles dissolve very water insoluble compounds, such as fat-soluble vitamins, inside the aggregates. This approach avoids the use of organic solvents in determining these vitamins and offers the possibility of analysing fat- and water-soluble vitamins simultaneously. The method has been validated in terms of detection limit, cut-off limit, sensitivity, number of false positives, number of false negatives and uncertainty range. The detection limit is about μg L−1. The screening method was applied to different samples such as pharmaceuticals, juices and isotonic drinks.
Similar content being viewed by others
References
European community (2002) Official diary L 31, 01 Feb 2002, p 1
European community (2002) Official diary L 183, 12 July 2002, p 6756
Lehninger A (1981) Bioquímica. Omega, Barcelona, p 341
Mauri-Aucejo AR, Llobat-Estelles M, Marín-Saez R (2003) Anal Bioanal Chem 375(5):643–652
Pastor-Ferrer O, Mauri-Aucejo AR, Llobat-Estelles M (2000) Fresenius J Anal Chem 367:485–490
Ramos-Lledó P, Vera S, San Andrés MP (2001) Fresenius J Anal Chem 369:91–95
Utecht RE (1993) J Chem Educ 70:673–673
López TJ, Lage MA, Alvarez ME (1998) Fresenius J Anal Chem 362:341–343
Alwarthan AA, Aly FA (1998) Talanta 45:1131–1138
Ramos-Martos N, Molina-Díaz A, Navalón A, de Orbe-Payá I, Capitán-Vallvey LF (2000) J Pharma Biomed Anal 23:837–844
Escandar GM, Bystol AJ, Campiglia AD (2002) Anal Chim Acta 466:275–283
Pérez-Ruiz T, Martínez-Lozano C, Tomás V, Ibarra I (1992) Talanta 39:907–911
Xiang-Qun G, Jin-Gou X, Yu-Zhou W, Yi-Bin Z, Xian-Zhi H, Guo-Zhen C (1993) Anal Chim Acta 276:151–160
Chen Q, Li D, Yang H, Zhu Q, Zheng H, Xu J (1999) Analyst 124:771–775
Viñas P, López-Erroz C, Cerdán FJ, Campillo N (2000) Mikrochim Acta 134:83–87
Du J, Li Y, Lu J (2002) Talanta 57:661–665
García L, Blázquez S, San Andrés MP, Vera S (2001) Anal Chim Acta 434:193–199
Ruisánchez I, Trullols E, Rius FX. Validación de métodos analíticos cualitativos. http://www.quimica.urv.es/quimio/
Pulido A, Ruisánchez I, Bosqué R, Rius FX (2002) Anal Chim Acta 455:267–275
Simonet BM, Rios A, Valcárcel M (2004) Anal Chim Acta 516:67–74
Song R, Schlecht PC, Ashley K (2001) J Hazard Mater 83:29–39
Trullols E, Ruisánchez I, Rius FX (2004) Trends Anal Chem 23(2):137–145
Ríos A, Barceló D, Buidens L, Cárdenas S, Heydorn K, Karlberg B, Klemm K, Lendl B, Milman B, Neidhart B, Stephany RW, Townshend A, Zschunke A, Valcárcel M (2003) Accred Qual Assur 8:68–77
Gambart D, Cárdenas S, Gallego M, Valcárcel M (1998) Anal Chim Acta 366:93–102
Lázaro E, San Andrés MP, Vera S (2000) Anal Chim Acta 413:159–166
Acknowledgements
The authors are greatly acknowledge to the financial support of Comunidad de Madrid (project no 07G/0050/2003) and the Ministerio de Ciencia y Tecnología (project no REN2003-01248-HID)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
León-Ruiz, V., Vera, S. & San Andrés, M.P. Validation of a screening method for the simultaneous identification of fat-soluble and water-soluble vitamins (A, E, B1, B2 and B6) in an aqueous micellar medium of hexadecyltrimethylammonium chloride. Anal Bioanal Chem 381, 1568–1575 (2005). https://doi.org/10.1007/s00216-005-3137-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-005-3137-0