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Microemulsion electrokinetic chromatographic determination of bufadienolides in toad venom and in traditional Chinese medicine

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Abstract

A microemulsion electrokinetic chromatographic (MEEKC) method has been developed and validated for determination of resibufogenin and cinobufagin in toad venom and in traditional Chinese medicine prepared from the venom. The MEEKC method involved use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent, and butan-1-ol as co-solvent. To improve the separation, the effect of temperature and running buffer pH were evaluated. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w), and 10 mmol L−1 sodium tetraborate buffer, pH 9.2, and 298 nm as the detection wavelength) enabled useful and repeatable separation of the analytes.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No.20375045) and the Chinese Academy of Sciences under a ‘‘Hundreds of Talents Program’’ to Li Yongmin.

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Authors and Affiliations

  1. Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, People’s Republic of China

    Luo Xingping, Zhai Zongde, Zhao Yanfang, Chen Liren & Li Yongmin

  2. The Graduate School of Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China

    Luo Xingping, Zhai Zongde & Zhao Yanfang

  3. Gansu Province Institute for Drug Control, Lanzhou, 730000, People’s Republic of China

    Luo Xingping

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  1. Luo Xingping
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  2. Zhai Zongde
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  3. Zhao Yanfang
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  4. Chen Liren
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  5. Li Yongmin
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Correspondence to Luo Xingping.

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Xingping, L., Zongde, Z., Yanfang, Z. et al. Microemulsion electrokinetic chromatographic determination of bufadienolides in toad venom and in traditional Chinese medicine. Anal Bioanal Chem 384, 1254–1258 (2006). https://doi.org/10.1007/s00216-005-0251-y

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  • DOI: https://doi.org/10.1007/s00216-005-0251-y

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