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Human urine certified reference material CZ 6009: creatinine, styrene metabolites (mandelic acid and phenylglyoxylic acid)

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Abstract

The reference material was prepared by freeze-drying pooled urine samples obtained from healthy persons occupationally exposed to styrene. The concentrations of mandelic acid (MA), phenylglyoxylic acid (PGA), and hippuric acid (HA) in urine were determined by three modes of high-performance liquid chromatography (HPLC). For isochronous stability testing the urinary mandelic acid and phenylglyoxylic acid concentrations were followed over a 24-month period for a preliminary batch by use of HPLC. No changes of the concentration values were found. The creatinine concentration was stable for more than five years. Standard Reference Material NIST 914a Creatinine was used for traceability purposes for creatinine. Pure chemicals MA and PGA were used for traceability purposes. Control material ClinChek-Urine Control (Recipe) was analyzed simultaneously. The mean values of MA and PGA compare well with the means and fall within the control range of control samples. Results from homogeneity, stability, and traceability testing were evaluated using the statistical program ANOVA. The certified values and their uncertainties were evaluated from the results of interlaboratory comparisons, and homogeneity and stability tests. The values are unweighed arithmetical averages of accepted results and their uncertainties are combined uncertainties (coverage factor=1).

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Abbreviations

MA:

Mandelic acid

HA:

Hippuric acid

PGA:

Phenylglyoxylic acid

3-HBA:

3-Hydroxybenzoic acid

ANOVA:

Analysis of variance

CV:

Coefficient of variance

NIST:

National Institute of Standards and Technology

HPLC:

High-performance liquid chromatography

References

  1. Bardoděj Z, David A, Šedivec V, Škramovský S, Teisinger J (1980) (eds) Exposure tests in industrial toxicology. Avicenum, pp 39, 291, 308

  2. Guillemin MP, Bauer D (1979) Int Arch Occup Environ Health 44:249–263

    CAS  PubMed  Google Scholar 

  3. Cikrt M (1998) (eds) Manual of prevention in medical practice, National Health Programme. Fortuna, Prague, pp 118–131

  4. Narayanan S, Appleton HD (1980) Clin Chem 26:1119–1126

    CAS  PubMed  Google Scholar 

  5. Šperlingová I, Dabrowská L, Kučera J, Tichý M (1995) Fresenius J Anal Chem 352:87–91

    Google Scholar 

  6. Šperlingová I, Dabrowská L, Stránský V, Kučera J, Tichý M (2003) Accred Qual Assur 8:113–116

    Google Scholar 

  7. Angerer J, Schaller KH (1988) (eds) Analyses of hazardous substances in biological materials methods for biological monitoring, vol 2. VCH, Weinheim, pp 52–66

  8. Chemicals and Instruments, Munich, Germany (2003) ClinChek-Control (Urine Control lyophilized) for Toxic Organic Compounds Recipe

  9. Kučera J, Faltejsek J (1995) Fresenius J Anal Chem 352:80–86

    Google Scholar 

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Acknowledgement

This work was supported by the Internal Grant Agency of the Ministry of Health of the Czech Republic (Grant No. NJ/6784–3).

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Authors and Affiliations

  1. National Institute of Public Health, Šrobárova 48, 100 42, Praha, Czech Republic

    I. Šperlingová, L. Dabrowská, V. Stránský & M. Tichý

  2. Nuclear Physics Institute, 250 68, Řež near Praha, Czech Republic

    J. Kučera

Authors
  1. I. Šperlingová
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  2. L. Dabrowská
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  3. V. Stránský
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  4. J. Kučera
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  5. M. Tichý
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Correspondence to I. Šperlingová.

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Šperlingová, I., Dabrowská, L., Stránský, V. et al. Human urine certified reference material CZ 6009: creatinine, styrene metabolites (mandelic acid and phenylglyoxylic acid). Anal Bioanal Chem 378, 1208–1212 (2004). https://doi.org/10.1007/s00216-003-2222-5

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  • DOI: https://doi.org/10.1007/s00216-003-2222-5

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