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Flow injection analysis: Rayleigh light scattering technique for total protein determination

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Abstract

A novel flow injection analysis (FIA) method with Rayleigh light scattering (RLS) detection was developed for the determination of total protein concentrations. This method is based on the weak intensity of RLS of bromothymol blue (BB) (3′,3″-dibromothymolsulfonephthalein) which can be enhanced by the addition of protein in weakly acidic solution. A common spectrofluorimeter was used as a detector. It was proved that the application of this method to quantify the total proteins in real samples by using bovine serum albumin was possible. The RLS signal was detected at λ ex=λ em=572 nm. The linear range was 7.0–70.0 μg mL−1, the detection limit was 3.75 μg mL−1, the reproducibility was 5.5% (n=7), and the sample throughput was 26 h−1.

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Authors and Affiliations

  1. FIA Laboratory, Department of Chemistry, Universidad Nacional del Sur, Av. Alem 1253, 8000 Bahía Blanca, Argentina

    Ezequiel Vidal, Miriam E. Palomeque, Adriana G. Lista & Beatriz S. Fernández Band

Authors
  1. Ezequiel Vidal
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  2. Miriam E. Palomeque
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  3. Adriana G. Lista
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  4. Beatriz S. Fernández Band
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Corresponding author

Correspondence to Beatriz S. Fernández Band.

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Vidal, E., Palomeque, M.E., Lista, A.G. et al. Flow injection analysis: Rayleigh light scattering technique for total protein determination. Anal Bioanal Chem 376, 38–41 (2003). https://doi.org/10.1007/s00216-003-1877-2

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  • DOI: https://doi.org/10.1007/s00216-003-1877-2

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