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One-step cleanup for PAH residue analysis in plant matrices using size-exclusion chromatography

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Abstract

A new one-step cleanup procedure, based on size-exclusion chromatography (SEC), usable for the extracts from accelerated solvent extraction (ASE), Soxhlet extraction, or ultrasonic extraction (USE), is described. The method is suitable for the determination of polycyclic aromatic hydrocarbons (PAHs), especially from very complicated plant matrices (e.g. pine needles, deciduous leaves, mosses). The main improvement compared with previous conventional procedures is that analyte peaks barely overlap with matrix peaks in the chromatograms and that it is a very rapid and simple one-step procedure with clearly improved analytical performance. Essential advantages of this SEC procedure are the sharper GC–MS chromatograms for the PAH fraction at retention times between 9.2 and 12.0 min, distinctly separated substance peaks resulting in better analysis, shorter running times, and lower solvent consumption.

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Authors and Affiliations

  1. Department of Chemical Ecotoxicology, UFZ Centre for Environmental Research Leipzig-Halle, Permoserstr. 15, 04318, Leipzig, Germany

    A. Hubert, K.-D. Wenzel & G. Schüürmann

  2. Department of Analytical Chemistry, UFZ Centre for Environmental Research Leipzig-Halle, Permoserstr. 15, 04318, Leipzig, Germany

    P. Popp

  3. Institute of Analytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, Linnéstrasse 3, 04103, Leipzig, Germany

    W. Engewald

Authors
  1. A. Hubert
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  2. P. Popp
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  3. K.-D. Wenzel
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  4. W. Engewald
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  5. G. Schüürmann
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Correspondence to A. Hubert.

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Hubert, A., Popp, P., Wenzel, KD. et al. One-step cleanup for PAH residue analysis in plant matrices using size-exclusion chromatography. Anal Bioanal Chem 376, 53–60 (2003). https://doi.org/10.1007/s00216-003-1858-5

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  • DOI: https://doi.org/10.1007/s00216-003-1858-5

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