Abstract
Lipid peroxidation (LPO), measured as thiobarbituric acid reactive substances (TBARS), was evaluated in lungs of rats 24 h after intraperitoneal injection of 50, 250, and 1000 μg Cd/kg body weight as CdCl2. In order to gain some insight into possible causative factors responsible for these oxidative phenomena, the redox-active elements iron (Fe) and copper (Cu), and total lung protein content (an indication of pulmonary inflammatory processes) were also measured. Results obtained demonstrate a similar dose-related, non-linear evolution of total lung TBARS and total lung protein as a function of increasing lung Cd concentrations. Standardization of total lung TBARS to lung protein content further resulted in a linear relationship with lung Cd concentrations, thus suggesting a possible cause-effect relationship between these parameters. No statistically significant association was observed between the dose-related evolution of lung TBARS, and iron (Fe) and copper (Cu) after Cd exposure. The results obtained provide support for the possible involvement of inflammatory phenomena as the most likely events responsible for the generation of LPO in lung tissue following acute exposure to Cd salts.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amoruso MA, Witz G, Goldstein BD (1982) Enhancement of rat and human phagocyte superoxide anion radical production by cadmium in vitro. Toxicol Lett 10: 133–138
Buckley BJ, Bassett DJP (1987) Pulmonary cadmium oxide toxicity in the rat. J Toxicol Environ Health 21: 233–250
Cross CE, Omaye ST, Rifas DC, Hasegawa GK, Reddy KA (1979) Biochemical effects of intratracheal instillation of cadmium chloride on rat lung. Biochem Pharmacol 28: 381–388
Driscoll KE, Maurer JK, Poynter J, Higgins J, Asquith T, Sue Miller N (1992) Stimulation of rat alveolar macrophage fibronectin release in a cadmium chloride model of lung injury and fibrosis. Toxicol Appl Pharmacol 116: 30–37
Gabor S, Anca Z, Bordas E (1978) Cadmium-induced lipid peroxidation in kidney and testes. Effect of zinc, copper. Rev Roum Biochem 15[2]: 113–117
Grose EC, Richards JH, Jaskot RH, Ménache MG, Graham JA, Dauterman WC (1987) A comparative study of the effects of inhaled cadmium chloride and cadmium oxide: pulmonary response. J Toxicol Environ Health 21: 209–232
Halliwell B, Guteridge JMC (1985) The importance of free radicals and catalytic metal ions in human diseases. Mol Aspects Med 8: 89–193
Harvey MJ, Klaassen CD (1983) Interaction of metals and carbon tetrachloride on lipid peroxidation and hepatotoxicity. Toxicol Appl Pharmacol 71: 316–322
Hirano S, Tsukamoto N, Kobayashi E, Suzuki KT (1989a) Toxicity of cadmium oxide instilled into the rat lung. I. Metabolism of cadmium oxide in the rat lung and its effects on essential elements. Toxicology 55: 15–24
Hirano S, Tsukamoto N, Higo S, Suzuki KT (1989b) Toxicity of cadmium oxide instilled into the rat lung. II. Inflammatory responses in broncho-alveolar lavage fluid. Toxicology 55: 25–35
Jamall IS, Sprowls JJ (1987) Effects of cadmium and dietary selenium on cytoplasmic and mitochondrial antioxidant defence systems in the heart of rats fed high dietary copper. Toxicol Appl Pharmacol 87: 102–110
Kobrle V, Hurych J, Cikrt M, Jones MM (1991) Sodium bis(hydroxyethyl)dithiocarbamate reduces acute lung tissue damage induced by cadmium in rats. Fundam Appl Toxicol 16: 733–741
Koizumi T, Li ZG (1992) Role of oxidative stress in single-dose, cadmium-induced testicular cancer. J Toxicol Environ Health 37: 25–36
Kojima S, Ishihara N, Hirukawa H, Kiyozumi M (1990) Effect of n-benzyl-d-glucamine dithiocarbamate on lipid peroxidation in testes of rats treated with cadmium. Res Commun Chem Pathol Pharmacol 67[2]: 259–269
Kornburst DJ, Mavis RD (1980) Relative susceptibilities of microsomes of lung, heart, liver, kidney, brain, and testes to lipid peroxidation: correlation with vitamin E content. Lipids 15: 315–322
Manca D, Ricard AC, Vincent R, Trottier B, Van Tra H, Chevalier G (1992a) Lipid peroxidation in rats subacutely exposed to a low concentration of aerosolized cadmium chloride. Inhal Toxicol 3: 419–433
Manca D, Lefebvre M, Trottier B, Laparé S, Ricard AC, Van Tra H, Chevalier G (1992b) Micro method for the determination of cadmium in tissues and slurried samples by use of flameless atomic absorption spectrometry. Microchem J 46: 249–258
Manca D, Ricard AC, Trottier B, Chevalier G (1990) In vitro susceptibilities of rat tissues to cadmium-induced lipid peroxidation: comparison of evolved hydrocarbons and thiobarbituric acid reactive substances. In Vitro Toxicol 3[3]: 255–323
Manca D, Ricard AC, Trottier B, Chevalier G (1991a) Studies on lipid peroxidation in rat tissues following administration of low and moderate doses of cadmium chloride. Toxicology 67: 303–323
Manca D, Ricard AC, Trottier B, Chevalier G (1991b) In vitro and in vivo responses of rat tissues to cadmium-induced lipid peroxidation. Bull Environ Contam Toxicol 46: 929–936
Martin FM, Witschi HP (1985) Cadmium-induced lung injury: cell kinetics and long-term effects. Toxicol Appl Pharmacol 80: 215–227
Muller L (1986a) Consequences of cadmium toxicity in rat hepatocytes: effects of cadmium on the glutathione-peroxidase system. Toxicol Lett 30: 259–265
Muller L (1986b) Consequences of cadmium toxicity in rat hepatocytes: mitochondrial dysfunction and lipid peroxidation. Toxicology 40: 285–295
Muller L, Ohnesorge FK (1984) Cadmium-induced alteration of the energy level in isolated hepatocytes. Toxicology 31: 297–306
Nayini NR, White BC, Aust SD, Huang RR, Indrieri NJ, Evans AT, Bialek H, Jacobs WA, Komara J (1985) Post resuscitation iron delocalization and malondialdehyde production in the brain following prolonged cardiac arrest. J Free Rad Biol Med 1: 111–116
Ochi T, Ohsawa M (1985) Participation of active oxygen species in the induction of chromosomal aberrations by cadmium chloride in cultured Chinese hamster cells. Mutat Res 143: 137–142
Oetken G, Beyersmann D (1991) Metallothionein or cadmium and GSH-induce single strand breaks in isolated DNA. Interrelation Between Chemistry and Biology, Fourth IAEAC Workshop on Toxic Metal Compounds, Les Diablerets, Switzerland. Metal Compounds in Environment and Life 4: 287–292
Ohkawa H, Ohishi N, Yagi K (1979) Assay of lipid peroxides in animal tissues by thiobarbituric reaction. Anal Biochem 95: 351–358
Ricard AC, Guilbaud R, Boileau S, Van Tra H, Chevalier G Absence of in vivo lipid peroxidation in CdCl2-treated rats as measured by exhaled pentane. Toxicol Lett (in press)
Stacey NH, Kappus H (1982) Comparison of methods of assessment of metal-induced lipid peroxidation in isolated rat hepatocytes. J Toxicol Environ Health 9: 277–285
Stacey NH, Cantilena LR Jr, Klaassen CD (1980) Cadmium toxicity and lipid peroxidation in isolated rat hepatocytes. Toxicol Appl Pharmacol 53: 470–480
Vincent R, Nadeau D (1983) A micromethod for the quantitation of cellular protein with the Coomassie brilliant blue dye-binding assay. Anal Biochem 135: 355–362
Vincent R, Boudreau J, Nadeau D, Fournier M, Krzystyniak K, Trottier B, Chevalier G (1989) Lipid peroxidation in rat lungs following an acute inhalation exposure to cadmium chloride. J Aerossol Med 2[4]: 349–363
Waalkes MP, Coogan TP, Barter RA (1992) Toxicological principles of metal carcinogenesis with special emphasis on cadmium. Crit Rev Toxicol 22[3,4]: 175–201
Wormser U, Nir I (1988) Effect of age on cadmium-induced metallothionein synthesis in the rat. Arch Toxicol 62: 392–394
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Manca, D., Ricard, A.C., Van Tra, H. et al. Relation between lipid peroxidation and inflammation in the pulmonary toxicity of cadmium. Arch Toxicol 68, 364–369 (1994). https://doi.org/10.1007/s002040050083
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s002040050083