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PARP-1 expression and activity in primary human lung cells

  • Organ Toxicity and Mechanisms
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Abstract

Activation of poly(ADP-ribose) polymerase-1 (PARP-1) in response to DNA damage is an important mechanism to keep homeostasis or to trigger apoptosis. The expression and function of (PARP-1) was studied in primary cells cultured from human lung. Normal human bronchial epithelial cells (NHBEC) and peripheral lung cells (PLC) from lung cancer patients were grown as explant cultures and were followed over a period of 12 weeks. PARP-1 protein was expressed in all explant cultures from bronchial epithelium. The levels of PARP protein differed between individuals by a factor of 2.3 in the first explant. Three cases were followed for more than 100 days. The expression levels varied intra-individually by a factor of 1.3–1.4 over this time period. PARP-1 activity was determined immunohistochemically after induction of DNA damage with H2O2 (0.05–0.3 mM, 5 min). The fluorescence signal for ADP-ribose polymers attached to chromatin proteins correlated well with the concentration of H2O2. PARP-1 activity differed by a factor of 3.1 in NHBECs obtained from the first generation of explants from 11 cases. PARP-1 activity is present in NHBECs until the 8th and in PLCs until the 12th week and declined to about half of the start level. Primary cultures of NHBECs and PLC are suitable to study the effect of external factors on PARP-1 expression and function.

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Acknowledgments

This study was supported by a grant from Deutsche Forschungsgesellschaft (DFG, Graduiertenkolleg 416) and the Institute for Science and Health, St Louis, USA (IFSH).

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Authors and Affiliations

  1. Institute of Environmental Toxicology, Martin Luther University Halle Wittenberg, Franzosenweg 1a, 06097, Halle, Saale, Germany

    Mohamed Ahmad, Abdelrahman Torky, Felix Glahn & Heidi Foth

  2. Department of Cardiothoracic Surgery, Martin-Luther University Halle Wittenberg, 06120, Halle, Germany

    Robert J. Scheubel

Authors
  1. Mohamed Ahmad
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  2. Abdelrahman Torky
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  3. Felix Glahn
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  4. Robert J. Scheubel
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  5. Heidi Foth
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Corresponding author

Correspondence to Heidi Foth.

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Part of the doctoral thesis of M. Ahmad (MD).

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Ahmad, M., Torky, A., Glahn, F. et al. PARP-1 expression and activity in primary human lung cells. Arch Toxicol 85, 669–679 (2011). https://doi.org/10.1007/s00204-010-0604-y

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  • DOI: https://doi.org/10.1007/s00204-010-0604-y

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