Abstract
Betel quid chewing is a general oral habit in Taiwan, India, southeastern Asian and South Africa with or without the additive of tobacco, alcohol or lime. In this study, the tumor-promoting neoplastic transformation effect of Lime-Piper betel quid (LPB) was examined on JB6 cells. The treatment of LPB at a high dose (1.0 mg/ml) for over 5 days or at lower doses (0.1, 0.5 mg/ml) for over 15 days induced the formation of transformed foci. The transformed cells showed the characteristics of colony formation in soft agar, higher growth rate and multilayer on culture dish. A two-fold induction of the protein levels of c-fos and c-jun proto-oncogenes was observed in the cells from the 50th passage (Cl1/p50, Cl2/p50 and Cl3/p50), suggesting that LPB-transformed cells were oncogenic. In addition, the LPB-transformed cells possessed an elevated level of c-Myc and an increased cell population distributed in the S phase of the cell cycle. These results demonstrated the promotion effect of LPB and indicate that it could be a tumor promoter.
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Abbreviations
- ATCC:
-
American type culture collection
- BQ:
-
betel quid
- IPB:
-
inflorescence of Piper betel
- LPB:
-
lime-piper betel quid
- MEM:
-
minimal essential medium
- RIPA buffer:
-
150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS and 50 mM Tris base
- OSF:
-
oral submucous fibrosis
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This work was supported by a National Science Council Grant (NSC91-2745-B-040-001).
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Lin, MH., Wang, CJ., Huang, HP. et al. The tumorigenic characteristics of Lime-Piper betel quid-transformed JB6 cells. Arch Toxicol 78, 167–173 (2004). https://doi.org/10.1007/s00204-003-0524-1
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DOI: https://doi.org/10.1007/s00204-003-0524-1