Abstract
In this study, three cold-tolerant phenol-degrading strains, Pseudomonas veronii Ju-A1 (Ju-A1), Leifsonia naganoensis Ju-A4 (Ju-A4), and Rhodococcus qingshengii Ju-A6 (Ju-A6), were isolated. All three strains can produce cis, cis-muconic acid by ortho-cleavage of catechol at 12 ℃. Response surface methodology (RSM) was used to optimize the proportional composition of low-temperature phenol-degrading microbiota. Degradation of phenol below 160 mg L−1 by low-temperature phenol-degrading microbiota followed first-order degradation kinetics. When the phenol concentration was greater than 200 mg L−1, the overall degradation trend was in accordance with the modified Gompertz model. The experiments showed that the microbial agent (three strains of low-temperature phenol-degrading bacteria were fermented separately and constructed in the optimal ratio) could completely degrade 200 mg L−1 phenol within 36 h. The above construction method is more advantageous in bio-enhanced treatment of actual wastewater. Through the construction of microbial agents to enhance the degradation effect of phenol, it provides a feasible scheme for the biodegradation of phenol wastewater at low temperature and shows good application potential.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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This work was financially supported by the Fundamental Research Funds for the Central Universities (No. DUT22LAB131).
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YY: investigation, experimental design, methodology, writing—original draft. YZ: conceptualization, formal analysis, supervision. CL: software, validation. ZS: data curation. RZ: investigation. JZ: review and editing.
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Yang, Y., Zhang, Y., Liu, C. et al. Low-temperature phenol-degrading microbial agent: construction and mechanism. Arch Microbiol 205, 193 (2023). https://doi.org/10.1007/s00203-023-03532-w
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DOI: https://doi.org/10.1007/s00203-023-03532-w