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Ziziphus spina-christi extract-stabilized novel silver nanoparticle synthesis for combating Fusarium oxysporum-causing pepper wilt disease: in vitro and in vivo studies

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Abstract

The novelty of the present study is studying the ability of aqueous Ziziphus spina-christi leaves’ extract (ZSCE) to produce eco-friendly and cost-effective silver nanoparticles (Ag NPs) against Fusarium wilt disease. Phytochemical screening of ZSCE by HPLC showed that they contain important antimicrobial substances such as Rutin, Naringin, Myricetin, Quercetin, Kaempferol, Hesperidin, Syringeic, Eugenol, Pyrogallol, Gallic and Ferulic. Characterization methods reveal a stable Ag NPs with a crystalline structure, spherical in shape with average particle size about 11.25 nm. ZSCE and Ag NPs showed antifungal potential against F. oxysporum at different concentrations with MIC of Ag NPs as 0.125 mM. Ag NPs treatment was the most effective, as it gave the least disease severity (20.8%) and the highest protection rate (75%). The application of ZSCE or Ag NPs showed a clear recovery, and its effectiveness was not limited for improving growth and metabolic characteristics only, but also inducing substances responsible for defense against pathogens and activating plant immunity (such as increasing phenols and strong expression of peroxidase and polyphenol oxidase as well as isozymes). Owing to beneficial properties such as antifungal activity, and the eco-friendly approach of cost and safety, they can be applied in agricultural field as novel therapeutic nutrients.

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Authors and Affiliations

  1. Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 13759, Egypt

    Amer M. Abdelaziz, Muhammad I. Haggag & Mohamed S. Attia

  2. Agricultural Biochemistry Department, Faculty of Agriculture, Al-Azhar University, Cairo, 13759, Egypt

    Mohammed A. Elshaer & Mohamed A. Abd-Elraheem

  3. Microbiology and Immunology Department, Faculty of Pharmacy, Galala University, New Galala City, Suez, Egypt

    Omar M Omar M Ali & Gharieb S. El-Sayyad

  4. Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt

    Gharieb S. El-Sayyad

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  1. Amer M. Abdelaziz
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  3. Mohamed A. Abd-Elraheem
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  4. Omar M Omar M Ali
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AMA: suggested the research topic, investigated the article, planned the research methodology, wrote the original draft, and participated in data representation and article revising and editing. MAE: suggested the research topic, investigated the article, planned the research methodology, wrote the original draft, and participated in data representation and article revising and editing. MAA: suggested the research topic, investigated the article, planned the research methodology, wrote the original draft, and participated in data representation and article revising and editing. GSE: suggested the research topic, investigated the article, planned the research methodology, drew the figures, wrote the original draft, and participated in data representation and article revising and editing. MSA: suggested the research topic, investigated the article, planned the research methodology, wrote the original draft, and participated in data representation and article revising and editing. All the authors read and approved the article.

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Correspondence to Gharieb S. El-Sayyad.

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Abdelaziz, A.M., Elshaer, M.A., Abd-Elraheem, M.A. et al. Ziziphus spina-christi extract-stabilized novel silver nanoparticle synthesis for combating Fusarium oxysporum-causing pepper wilt disease: in vitro and in vivo studies. Arch Microbiol 205, 69 (2023). https://doi.org/10.1007/s00203-023-03400-7

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