Abstract
Strain W712T was isolated from rhizosphere soil of Nicotiana tabacum L. collected from Kunming, south-west China. Cells were Gram-staining negative, aerobic, motile and rod shaped. The isolate grew at 20–45 °C (optimum 30 °C), pH 6.0–8.0 (optimum pH 7.0) and in the presence of up to 3.0% (w/v) NaCl (optimum 1%, w/v). Ubiquinone-10 was the only respiratory quinone type. Polar lipids contained diphosphatidylglycerol, phosphatidylmehtylethanolamine, phosphatidylglycerol, phosphatidylcholine and an unidentified aminolipid. The major fatty acids were detected as summed feature 8 (C18:1 ω7c or C18:1 ω6c), summed feature 3 (C16:1 ω7c or C16:1 ω6c) and C18:1 2OH. The genomic DNA G + C content was 68.7%. The ANI values were 94.3%, 93.3% and 93.6% between Azospirillum baldaniorum Sp245T, Azospirillum brasilense ATCC 49958T, Azospirillum formosense CC-Nfb-7T and strain W712T, respectively, which were lower than the prokaryotic species delineation threshold of 95.0–96.0%. The digital DNA–DNA hybridization values between A. baldaniorum Sp245T, A. brasilense ATCC 49958T, A. formosense CC-Nfb-7T and strain W712T indicated that the candidate represents a novel genomic species. According to the phenotypic and genotypic characteristics, we propose that strain W712T warrants the assignment to a novel species, for which the name Azospirillum tabaci sp. nov. (type strain W712T = CGMCC 1.18567T = KCTC 82186T) is proposed.
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Acknowledgements
The authors are grateful to Takuji Kudo (JCM, Japan) for providing the reference strains.
Funding
This research was funded by China Tobacco Yunnan Industrial Corp, (Grant No. 2020CL04) and Natural Science Foundation of Guangdong Province, China (No. 2016A030312003).
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GD and WJL designed research and project outline. YQD, XKZ, NH, SQG performed isolation, deposition, and identification. XKZ, LMD, XFL and YMS performed fatty acids analysis. All authors read and approved the final manuscript.
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Duan, YQ., Zhou, XK., Habib, N. et al. Azospirillum tabaci sp. nov., a bacterium isolated from rhizosphere soil of Nicotiana tabacum L. Arch Microbiol 204, 80 (2022). https://doi.org/10.1007/s00203-021-02688-7
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DOI: https://doi.org/10.1007/s00203-021-02688-7