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Genomic insight for algicidal activity in Rhizobium strain AQ_MP

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Abstract

Occurrence of Harmful Algal Blooms (HABs) creates a threat to aquatic ecosystem affecting the existing flora and fauna. Hence, the mitigation of HABs through an eco-friendly approach remains a challenge for environmentalists. The present study provides the genomic insights of Rhizobium strain AQ_MP, an environmental isolate that showed the capability of degrading Microcystis aeruginosa (Cyanobacteria) through lytic mechanisms. Genome sequence analysis of Rhizobium strain AQ_MP unraveled the algal lytic features and toxin degradative pathways in it. Functional genes of CAZymes such as glycosyltransferases (GT), glycoside hydrolases (GH), polysaccharide lyases (PL) which supports algal polysaccharide degradation (lysis) were present in Rhizobium strain AQ_MP. Genome analysis also clarified the presence of the glutathione metabolic pathway, which is the biological detoxification pathway responsible for toxin degradation. The conserved region mlrC, a microcystin toxin-degrading gene was also annotated in the genome. The study illustrated that Rhizobium strain AQ_MP harbored a wide range of mechanisms for the lysis of Microcystis aeruginosa cells and its toxin degradation. In future, this study finds promiscuity for employing Rhizobium strain AQ_MP species for bioremediation, based on its physiological and genomic analysis.

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Acknowledgements

The authors are grateful to Director, CSIR-NEERI (KRC No: CSIR-NEERI/KRC/2020/Nov/EBGD/4), for providing all the necessary infrastructure required for the experimentation. The authors also thank DBT (BT/PR16149/NER/95/85/2015 dated 19/01/2017) for the funding.

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  1. Environmental Biotechnology and Genomics Division, CSIR- National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, 440 020, India

    Mili Pal, Hemant J. Purohit & Asifa Qureshi

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Mili Pal & Asifa Qureshi

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  1. Mili Pal
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Correspondence to Asifa Qureshi.

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Pal, M., Purohit, H.J. & Qureshi, A. Genomic insight for algicidal activity in Rhizobium strain AQ_MP. Arch Microbiol 203, 5193–5203 (2021). https://doi.org/10.1007/s00203-021-02496-z

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