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Streptomyces boluensis sp. nov., isolated from lake sediment

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Abstract

A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain YC537T, was isolated from lake sediment collected from Yenicaga Lake, Bolu, Turkey, and subjected to a polyphasic taxonomic approach. The organism had phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis of strain YC537T showed that it is closely related to the type strain of Streptomyces ziwulingensis F22T (97.9% sequence similarity), Streptomyces tauricus JCM 4837 T (97.7%) and Streptomyces beijiangensis NBRC 100044 T (97.6%). The cell wall of the strain contained LL-diaminopimelic acid and the cell-wall sugars were glucose, galactose and ribose. The major phospholipids of strain YC537T were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H6) and MK-9(H8). The major cellular fatty acids were iso-C16:0, iso-C14:0, anteiso-C15:0 and iso-C15:0. Consequently, strain YC537T is considered to represent a novel species in the genus Streptomyces, for which the name Streptomyces boluensis sp. nov. is proposed. The type strain is YC537T (= KCTC 39750 T = DSM 102303 T).

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Acknowledgements

Genome sequencing was provided by MicrobesNG (https://www.microbesng.uk). We gratefully acknowledges Cengiz Nigiz from Ondokuz Mayis University for help to us in obtaining the sediment samples.

Funding

This research was supported by Amasya University (AU), project no. FMB-BAP 15-0147.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, 55139, Samsun, Turkey

    Ali Tokatli

  2. Department of Biology, Faculty of Science and Arts, Amasya University, 05000, Amasya, Turkey

    Ali Tokatli

  3. Department of Pre-School Education, Faculty of Education, Amasya University, 05100, Amasya, Turkey

    Onder Idil

  4. Department of Medical Laboratory Techniques, Vocational School of Health Services, Sinop University, 57000, Sinop, Turkey

    Aysel Veyisoglu

  5. Department of Molecular Biology and Genetics, Faculty of Science and Arts, Ondokuz Mayis University, 55139, Samsun, Turkey

    Hayrettin Saygin & Nevzat Sahin

  6. Department of Biology, Faculty of Science, Eskisehir Technical University, 26555, Eskisehir, Turkey

    Kiymet Guven

  7. Division of Science Education, Department of Mathematics and Science Education, Gazi Faculty of Education, Gazi University, 06500, Ankara, Turkey

    Demet Cetin

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  1. Ali Tokatli
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Contributions

NS and OI designed the study. AT, AV and HS performed the laboratory experiments. KG contributed to the fatty acids determination. DC contributed to electron microscopy. NS and AV wrote the paper. All authors read and approved the manuscript.

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Correspondence to Onder Idil or Nevzat Sahin.

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This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

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Communicated by Erko Stackebrandt.

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203_2020_1901_MOESM1_ESM.jpg

Fig. S1. Scanning electron micrograph of strain YC537T grow on oatmeal agar (ISP3) medium at 28 °C for 32 days. Bar, 5 μm 1 (JPG 92 kb)

203_2020_1901_MOESM2_ESM.jpg

Fig. S2. The phospholipids of strain YC537. Diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphoglycolipid (PGL), three unidentified aminolipids (AL) and two unidentified phospholipids (PL). Solvent used were CHCl3: CH3OH: distilled water (65:25:4) for the first run and CHCl3: glacial acetic acid: CH3OH: distilled water (80:12:15:4) for the second run (JPG 220 kb)

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Tokatli, A., Idil, O., Veyisoglu, A. et al. Streptomyces boluensis sp. nov., isolated from lake sediment. Arch Microbiol 202, 2303–2309 (2020). https://doi.org/10.1007/s00203-020-01901-3

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