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Ornithinibacillus caprae sp. nov., a moderate halophile isolated from the hides of a white goat

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Abstract

A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China. The isolate grew optimally at 30 °C, at pH 7 and with 5–10% (w/v) NaCl. The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0. The peptidoglycan amino acid type was determined to be A4β, containing l-ornithine and d-aspartic as diagnostic amino acids. The phospholipids were dominated by diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophospholipid and two unidentified phospholipids. Genome sequencing resulted in a genome size of 4.0 Mbp and a DNA G + C content of 35.9 mol%. Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity). The ANI and dDDH values between strain L9T and the closely related species were 69.8–76.1% and 13.0–20.5%, respectively. On the basis of the data presented, strain L9T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus caprae sp. nov. is proposed. The type strain is L9T (= KCTC 43176T = CGMCC 1.17659T).

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Abbreviations

LB:

Luria–Bertani

MK-7:

Menaquinone-7

Orb :

Ornithinibacillus

Ocb :

Oceanobacillus

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

MEGA:

Molecular evolutionary genetics analysis

KCTC:

Korean Collection for Type Cultures

CGMCC:

China General Microbiological Culture Collection Center

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Acknowledgements

We thank Prof. Aharon Oren for his valuable help with naming the species. This work is supported by the National Key Research and Development Program of China (2017YFB0308401).

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Authors and Affiliations

  1. College of Biomass Science and Engineering, Sichuan University, Chengdu, People’s Republic of China

    Xiaoguang Li, Shihao Zhang, Longzhan Gan, Chufan Cai, Yongqiang Tian & Bi Shi

  2. Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu, People’s Republic of China

    Xiaoguang Li, Shihao Zhang, Longzhan Gan, Chufan Cai, Yongqiang Tian & Bi Shi

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  1. Xiaoguang Li
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  2. Shihao Zhang
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  3. Longzhan Gan
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  4. Chufan Cai
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  6. Bi Shi
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Contributions

XL performed the predominant experiments, analysed the data and drafted the manuscript. SZ and CC were responsible for collecting samples. LG contributed to the cell wall peptidoglycan amino acids determination. YT and BS conceived the study and supervised the study. All the authors discussed the results and revised the manuscript.

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Correspondence to Yongqiang Tian.

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Communicated by Erko Stackebrandt.

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Li, X., Zhang, S., Gan, L. et al. Ornithinibacillus caprae sp. nov., a moderate halophile isolated from the hides of a white goat. Arch Microbiol 202, 1469–1476 (2020). https://doi.org/10.1007/s00203-020-01855-6

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  • DOI: https://doi.org/10.1007/s00203-020-01855-6

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