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Description of Deinococcus oregonensis sp. nov., from biological soil crusts in the Southwestern arid lands of the United States of America

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Abstract

Biological soil crusts are distinct habitats, harbor unique prokaryotic diversity and gave an impetus to isolate novel species. In the present study, a pink-pigmented bacterium, (OR316-6T), was isolated from biological soil crusts using oligotrophic BG11-PGY medium. Strain OR316-6T was Gram-positive, short rods, non-motile and non-spore forming. Cells were positive for catalase, oxidase and β-galactosidase and negative for most of the enzymatic activities. The major fatty acids present were C16:0, C17:0, and C16:1ω7c and contained MK-8 and MK-10 as the predominant menaquinones. The cell wall peptidoglycan was of A3β variant with l-ornithine as the diamino acid. Based on the above characteristics, strain OR316-6T was assigned to the genus Deinococcus. The phylogenetic analysis indicated that strain OR316-6T was closely related to D. aquatilis DSM 23025T with a 16S rRNA gene similarity of 99.3 % and clustered with a bootstrap value of 100 %. DNA–DNA similarity between strain OR316-6T and D. aquatilis DSM 23025T was 37.0 % indicating that strain OR316-6T was a novel species. Further, DNA fingerprinting of stains OR316-6T and D. aquatilis DSM 23035T demonstrated that both strains were related to each other with a similarity coefficient of only 0.32 and supported the species status to strain OR316-6T. In addition, phenotypic characteristics distinguished strain OR316-6T from D. aquatilis DSM 23025T. Based on the cumulative differences, strain OR316-16T exhibited with its closely related species, it was identified as a novel species and proposed the name Deinococcus oregonensis sp. nov. The type strain is D. oregonensis sp. nov. (OR316-6T = JCM 13503T = DSM 17762T).

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Acknowledgments

This research was funded by the National Science Foundation Biotic Surveys and Inventories grant 0206711 to F. G-P.

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Authors and Affiliations

  1. School of Life Sciences, Arizona State University, Main Campus, Tempe, AZ, 85287-4501, USA

    Sathyanarayana Reddy Gundlapally & Ferran Garcia-Pichel

  2. CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500 007, India

    Sathyanarayana Reddy Gundlapally

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  1. Sathyanarayana Reddy Gundlapally
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  2. Ferran Garcia-Pichel
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Correspondence to Sathyanarayana Reddy Gundlapally.

Additional information

Communicated by Erko Stackebrandt.

GenBank/EMBL accession numbers for the 16S rRNA gene sequence of Deinococcus oregonensis sp. nov. (OR316-6T) is KT756665.

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Gundlapally, S.R., Garcia-Pichel, F. Description of Deinococcus oregonensis sp. nov., from biological soil crusts in the Southwestern arid lands of the United States of America. Arch Microbiol 199, 69–76 (2017). https://doi.org/10.1007/s00203-016-1273-x

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