Abstract
Classical methodologies for Salmonella detection may be too long in time to assure public safety. Presently, one of the fastest assays for Salmonella detection using the mini-VIDAS® system is the Easy Salmonella protocol. This assay, developed for food matrixes analysis, was here assessed for the applicability on the detection of these bacteria in transitional and saltwaters. The presence of Salmonella was detected in 4.2 % of the samples studied. In these transitional waters, the proposed protocol presented an efficiency of 79.1 %, due to a high false positive rate (20.8 %), and a false negative rate of 0 %—implying reducing analysis time, the use of enrichment broths, and making it more cost effective. Despite the multitude of samples nature, the method here described revealed to be an efficient and promising tool for transitional waters analysis.
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References
AFNOR (1994) Validation certification of the VIDAS® Salmonella dual selective enrichment for the rapid detection of Salmonella according to standard EN ISO 16140:2003 (Certificate No.: BIO-12/1 – 04/94 VIDAS® SLM - ref. 30702)
AFNOR (2005) Validation certificate for alternative analytical method according to standard EN ISO 16140:2003 (Certificate No.:BIO-12/16 – 09/05 VIDAS® Easy Salmonella – Ref. 30702)
AFNOR (2009) AFNOR validation following the EN ISO 16140 standard of the VIDAS® Easy Salmonella method (Certificate No.: BIO-12/16 – 09/05 VIDAS® Easy Salmonella – Ref. 30702)
APHA (2005) In: Eaton AD, Clesceri LS, Greenberg AE (eds) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association, Washington, DC
EC (2000) Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy. Official Journal of the European Communities, 22.12.2000, L327/1
EC (2006) Directive 2006/7/EC of the European Parliament and of the Council of 15 February 2006 concerning the management of bathing water quality and repealing Directive 76/160/EEC. Official Journal of the European Union, 4.3.2006, L64/37
EEC (1976) Council Directive of 8 December 1975 Concerning the Quality of Bathing Water (76/160/EEC). Official Journal of the European Communities. 5.2.1976, L/031
EPA (2001) Parameters of water quality: interpretation and standards. Environmental Protection Agency, Ireland
Ho BSW, Tam TY (2000) Rapid enumeration of Salmonella in environmental waters and wastewater. Wat Res 34(8):2397–2399
ISO 16140 (2003) Microbiology of food and animal feeding stuffs—protocol for the validation of alternative methods. International Organization for Standardization, Geneve
ISO 19250 (2010) Water quality—detection of salmonella species. International Organization for Standardization, Geneve
ISO 6222 (1999) Water quality—enumeration of culturable micro-organisms—colony count by inoculation in a nutrient agar culture medium. ISO, Geneve
ISO 6340 (1995) Water quality—detection and enumeration of Salmonella. International Organization for Standardization, Geneve
ISO 6579 (2002) Microbiology of food and animal feeding stuffs—horizontal method for the detection of Salmonella spp. International Organization for Standardization, Geneve
ISO, TR 6579–3 (2014) Microbiology of the food chain—horizontal method for the detection, enumeration and serotyping of Salmonella—part 3: Guidelines for serotyping of Salmonella spp. International Organization for Standardization, Geneve
Mansilha CR, Coelho CA, Reinas A, Moutinho A, Ferreira S, Pizarro C, Tavares A (2010) Salmonella: the forgotten pathogen: health hazards of compliance with European bathing water legislation. Mar Pollut Bull 60:819–826
Metodik L (1992) Ferskvandsbiologisk laboratorium. Kobenhawns Universitet, Akademisk Forlag, København
Parsons TR, Maita Y, Lalli CM (1984) A manual of chemical and biological methods for seawater analysis. Pergamon Press, Oxford
Schets FM, van Wijnen JH, Schijven JF, Schoon H, Roda Husman AM (2008) Monitoring of waterborne pathogens in surface waters in Amsterdam, The Netherlands, and the potential health risk associated with exposure to Cryptosporidium and Giardia in these waters. Appl Environ Microbiol 74:2069–2078
Strickland JDH, Parsons TR (1972) A practical handbook of seawater analysis. Bull Fish Res Board Can 167:71–80
ter Braak C, Smilauer P (1998) CANOCO reference manual and user’s guide to Canoco for windows—software for canonical community ordination (version 4). Microcomputer Power, Ithaca
Willey JM, Sherwood LM, Woolverton CJ (2008) In: Prescott L, Harley JP, Klein DA (eds) Microbiology, 7th edn. McGraw Hill, New York
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Communicated by Erko Stackebrandt.
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Rodrigues, M.J., Martins, K., Garcia, D. et al. Using the mini-VIDAS® Easy Salmonella protocol to assess contamination in transitional and coastal waters. Arch Microbiol 198, 483–487 (2016). https://doi.org/10.1007/s00203-016-1211-y
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DOI: https://doi.org/10.1007/s00203-016-1211-y