Skip to main content
Log in

Methodological considerations in measurement of bone mineral content

  • Original Article
  • Published:
Osteoporosis International Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.

References

  1. Boivin GY, Chavassieux PM, Santora AC, Yates J, Meunier PJ (2000) Alendronate increases bone strength by increasing the mean degree of mineralization of bone tissue in osteoporotic women. Bone 27:687–694

    Google Scholar 

  2. Arnold JS, Bartley MH, Tont SA, Jenkins DP (1966) Skeletal changes in aging and disease. Clin Orthop 49:17–38

    CAS  PubMed  Google Scholar 

  3. Boivin G, Baud C (1984) Microradiographic methods for calcified tissues. In: Dickson G (ed) Methods of calcified tissue preparation. Elsevier, Amsterdam, pp 391–411

  4. Meunier PJ, Boivin G (1997) Bone mineral density reflects bone mass but also the degree of mineralization of bone: therapeutic implications. Bone 21:373–377

    Article  CAS  PubMed  Google Scholar 

  5. Boivin G, Meunier P (2002) The degree of mineralization of bone tissue measured by computerized quantitative contact microadiography. Calcif Tissue Int 70:503–511

    Article  CAS  PubMed  Google Scholar 

  6. Reid SA, Boyde A (1987) Changes in the mineral density distribution in human bone with age: image analysis using backscattered electrons in the SEM. J Bone Miner Res 2:13–22

    PubMed  Google Scholar 

  7. Skedros JG, Bloebaum RD, Bachus KN, Boyce TM (1993) The meaning of gray levels in backscattered electron images of bone. J Biomed Mater Res 27:47–56

    CAS  PubMed  Google Scholar 

  8. Skedros JG, Bloebaum RD, Bachus KN, Boyce TM, Constantz B (1993) Influence of mineral content and composition on gray levels in backscattered electron images of bone. J Biomed Mater Res 27:57–64

    CAS  PubMed  Google Scholar 

  9. Boyde A, Jones S, Aerssens J, Dequeker J (1995) Mineral density quantitation of the human cortical iliac crest by backscattered electron image analysis: variations with age, sex and degree of osteoarthritis. Bone 16:619–627

    Article  CAS  PubMed  Google Scholar 

  10. Roschger P, Plenk H, Klaushofer K, Eschberger J (1995) A new scanning electron microscopy approach to the quantification of bone mineral distribution: backscattered electron image grey-levels correlated to calcium K-alpha-line intensitites. Scanning Microsc 9:75–88

    CAS  PubMed  Google Scholar 

  11. Roschger P, Fratzl P, Eschberger J, Klaushofer K (1998) Validation of quantitative backscattered electron imaging for the measurement of mineral density distribution in human bone biopsies. Bone 23:319–326

    Article  CAS  PubMed  Google Scholar 

  12. Bloebaum R, Skedros J, Vajda E, Bachus K, Constantz B (1997) Determining mineral content variations in bone using backscattered electron imaging. Bone 20:485–490

    Article  CAS  PubMed  Google Scholar 

  13. Nuzzo S, Peyrin F, Cloetens P, Bachurel J, Boivin G (2002) Quantification of the degree of mineralization of bone in three dimension using synchrotron radiation microtemography. Med Phys 29:2672–2681

    Google Scholar 

  14. Fratzl P, Groschner M, Vogl G, et al (1992) Mineral crystals in calcified tissues: a comparative study by SAXS. J Bone Miner Res 7:329–334

    CAS  PubMed  Google Scholar 

  15. Fratzl P, Schreiber S, Klaushofer K (1996) Bone mineralization as studied by small-angle x-ray scattering. Connect Tissue Res 34:247–254

    CAS  PubMed  Google Scholar 

  16. Paschalis EP, Betts F, DiCarlo E, Mendelsohn R, Boskey AL (1997) FTIR microspectroscopic analysis of normal human cortical and trabecular bone. Calcif Tissue Int 61:480–486

    Article  CAS  PubMed  Google Scholar 

  17. Camacho N, Rinnerthaler S, Paschalis E, et al (1999) Complementary information on bone ultrastruture from scanning small angle x-ray scattering and Fourier-transform infrared microspectroscopy. Bone 25:287–293

    Article  CAS  PubMed  Google Scholar 

  18. Glimcher M (1997) The nature of the mineral phase in bone. In: Avioli LV, Krane SM (eds) Metabolic bone disease. Academic Press, San Diego, pp 23–50

  19. Boivin G, Meunier P (2002) Effects of bisphosphonates on matrix mineralization. J Musculoskel Neuron Interact 2:538–543

    CAS  Google Scholar 

  20. Roschger P, Grabner B, Messmer P, et al (2001) Influence of intermittent PTH treatment on mineral distribution in the human ilium: a pair biopsy study before and after treatment. J Bone Min Res 17 (Suppl 1):S179

    Google Scholar 

  21. Roschger P, Rinnerthaler S, Yates J, et al (2001) Alendronate increases degree and uniformity of mineralization in cancellous bone and decreases the porosity in cortical bone of osteoporotic women. Bone 29:185–191

    Article  CAS  PubMed  Google Scholar 

  22. Nuzzo S, Lafage-Proust M, Martin-Badosa E, et al (2002) Synchrotron radiation microtomography allows the analysis of three-dimensional microarchitecture and degree of mineralization of human iliac crest biopsy specimens: effects of etidronate treatment. J Bone Miner Res 17:1372–1382

    CAS  PubMed  Google Scholar 

  23. Ott S, Oleksik A, Lu Y, Harper K, Lips P (2002) Bone histomorphometric and biochemical marker results of a 2-year placebo-controlled trial of raloxifene in postmenopausal women. J Bone Miner Res 17:341–348

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors express their gratitude to Arthur Santora and John Yates (Merck Research Laboratories, Rahway, NJ) for their constant support. The collaboration of Paul Lips (Amsterdam, The Netherlands), Susan Ott (Seattle, WA), Kristine Harper (Eli Lilly and Company, Indianapolis, IN), Isabelle Tupinon-Mathieu (Institut de Recherches Internationales Servier, Courbevoie, France) is acknowledged. The expert technical assistance of Delphine Farlay, Catherine Simi, and Annie Buffet (INSERM Unité 403, Faculté de Médecine R. Laennec, Lyon, France) is also gratefully acknowledged.

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boivin, G., Meunier, P.J. Methodological considerations in measurement of bone mineral content. Osteoporos Int 14 (Suppl 5), 22–28 (2003). https://doi.org/10.1007/s00198-003-1469-1

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00198-003-1469-1

Keywords

Navigation