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Pid3-I1 is a race-specific partial-resistance allele at the Pid3 blast resistance locus in rice

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Abstract

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The rice blast resistance QTL detected on chromosome 6 in MC276 is Pid3-I1, one of the multiple alleles at the Pid3 locus. Pid3-I1 shows race-specific partial resistance.

Abstract

Many of the quantitative trait loci (QTLs) for rice blast resistance reported to date remain unidentified. In the present study, we focused on qBRM6.2, a known blast-resistance QTL in experimental resistant rice line MC276. A CO39 near-isogenic line (NIL) carrying qBRM6.2 from MC276 was developed here, and we showed that qBRM6.2 resistance was partial but race specific to Japanese blast isolates using the NIL. Because defense genes in the NIL were expressed sooner than those in CO39 after inoculation with a blast isolate, qBRM6.2 resistance appeared to be an induced resistance. Next, we demonstrated that qBRM6.2 was located within a 123-kb interval on chromosome 6. Among the six genes annotated in the interval, only four genes appeared to be functional. Among these four, a polymorphism between CO39 and the NIL for qBRM6.2 at the amino acid sequence level was detected only in Os06g0330400 that encodes a fatty acid hydroxylase domain-containing protein and in Os06g0330100, the blast resistance locus Pid3, that encodes a nucleotide-binding site–leucine-rich repeat protein. Moreover, the allele at the Pid3 locus in the NIL was Pid3-I1, originally identified as a complete blast resistance gene in Kasalath. To clarify whether Pid3-I1 is qBRM6.2, we investigated the resistance phenotype of Pid3-I1 to Japanese isolates using Nipponbare transgenic lines that express Pid3-I1. The results showed that Pid3-I1 was a race-specific but partial-resistance allele at the Pid3 locus, suggesting strongly that Pid3-I1 is qBRM6.2. The discrepancy in the phenotype of Pid3-I1 between the present and previous reports is also discussed.

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Acknowledgements

This work was supported by JSPS KAKENHI Grant No. JP16K07549.

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Authors and Affiliations

  1. Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan

    Tsuyoshi Inukai, Saki Nagashima & Miyako Kato

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  1. Tsuyoshi Inukai
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  2. Saki Nagashima
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  3. Miyako Kato
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Correspondence to Tsuyoshi Inukai.

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Communicated by Mingliang Xu.

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Inukai, T., Nagashima, S. & Kato, M. Pid3-I1 is a race-specific partial-resistance allele at the Pid3 blast resistance locus in rice. Theor Appl Genet 132, 395–404 (2019). https://doi.org/10.1007/s00122-018-3227-y

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