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Fine mapping TaFLW1, a major QTL controlling flag leaf width in bread wheat (Triticum aestivum L.)

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Abstract

Introduction

Flag leaf width (FLW) is directly related to photosynthetic capacity and yield potential in wheat. In a previous study, Qflw.nau-5A controlling FLW was detected on chromosome 5A in the interval possessing Fhb5 for type I Fusarium head blight (FHB) resistance using a recombinant inbred line population derived from Nanda2419 × Wangshuibai.

Materials and methods

Qflw.nau-5A near-isogenic line (NIL) with the background of Mianyang 99-323 and PH691 was developed and evaluated. FLW inheritance was investigated using two F2 populations developed from crossing the Qflw.nau-5A NILs with their recurrent parents. One hundred ten and 28 recombinants, which included 10 and 5 types of recombinants, were identified from 2816 F2 plants with Mianyang 99-323 background and 1277 F2 plants with PH691 background, respectively, and phenotyped in field trials for FLW and type I FHB resistance. Deletion bin mapping was applied to physically map Qflw.nau-5A.

Results and conclusions

The introduction of Wangshuibai Qflw.nau-5A allele reduced the FLW up to 3 mm. In the F2 populations, Qflw.nau-5A was inherited like a semi-dominant gene, and was therefore designated as TaFLW1. The FLW of the recombinant lines displayed a distinct two-peak distribution. Recombinants with wider leaves commonly have Mianyang 99-323 or PH691 chromatin in the 0.2 cM Xwmc492-Xwmc752 interval that resided in the 5AL12-0.35–0.57 deletion bin, and recombinants with narrow leaves were Wangshuibai genotype in this interval. Phenotypic recombination between FLW and type I FHB resistance was identified, implying TaFLW1 was in close linkage with Fhb5. These results should aid wheat breeders to break the linkage drag through marker-assisted selection and assist in the map-based cloning of TaFLW1.

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Acknowledgments

This study was partially supported by ‘973’ program (2011CB100103), ‘863’ program (2012AA101105), NSFC programs (31000535, 31271711), Research Fund for the Doctoral Program of Higher Education of China program (20100097120042), Fundamental Research Funds for the Central Universities (KYZ201139), ‘111’ project B08025 and the PAPD project of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. The Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Centre and National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Shulin Xue, Feng Xu, Guoqiang Li, Yan Zhou, Musen Lin, Zhongxia Gao, Xiuhong Su, Xiaowu Xu, Ge Jiang, Shuang Zhang, Haiyan Jia, Zhongxin Kong, Lixia Zhang & Zhengqiang Ma

  2. College of Agricultural Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Shulin Xue, Feng Xu, Guoqiang Li, Yan Zhou, Musen Lin, Zhongxia Gao, Xiuhong Su, Xiaowu Xu, Ge Jiang, Shuang Zhang, Haiyan Jia, Zhongxin Kong, Lixia Zhang & Zhengqiang Ma

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  1. Shulin Xue
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Correspondence to Zhengqiang Ma.

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Communicated by C. Hackett.

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Xue, S., Xu, F., Li, G. et al. Fine mapping TaFLW1, a major QTL controlling flag leaf width in bread wheat (Triticum aestivum L.). Theor Appl Genet 126, 1941–1949 (2013). https://doi.org/10.1007/s00122-013-2108-7

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  • DOI: https://doi.org/10.1007/s00122-013-2108-7

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