Skip to main content
SpringerLink
Log in

Identification and mapping of a tiller inhibition gene (tin3) in wheat

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Tillering is one of the most important agronomic traits in cereal crops because tiller number per plant determines the number of spikes or panicles per plant, a key component of grain yield and/or biomass. In order to characterize the underlying genetic variation for tillering, we have isolated mutants that are compromised in tillering ability using ethyl methanesulphonate (EMS)-based mutagenesis in diploid wheat (Triticum monococcum subsp. monococcum). The tillering mutant, tiller inhibition (tin3) produces only one main culm compared to the wild type with many tillers. The monoculm phenotype of tin3 is due to a single recessive mutation. Genetic and molecular mapping in an F2 population of diploid wheat located the tin3 gene on the long arm of chromosome 3Am. One codominant RFLP marker Xpsr1205 cosegregated with tin3 in the F2 population. Physical mapping of PSR1205 in a set of Chinese Spring deletion lines of group-3 chromosomes placed the tin3 gene in the distal 10% of the long arm of chromosome 3A, which is a recombination-rich region in wheat. The implications of the mapping of tin3 on chromosome arm 3AmL are discussed with respect to putative orthologs of tin3 in the 3L colinear regions across various cereal genomes and other tillering traits in grasses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Adonina IG, Salina EA, Pestsova EG, Roder MS (2005) Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in the S genome. Genome 48:959–970

    Article  PubMed  CAS  Google Scholar 

  • Ahn S, Anderson JA, Sorrells ME, Tanksley SD (1993) Homoeologous relationships of rice, wheat and maize chromosomes. Mol Gen Genet 241:483–490

    Article  PubMed  CAS  Google Scholar 

  • Akhunov ED, Goodyear AW, Geng S, Qi LL, Echalier B, Gill BS, Miftahudin, Gustafson JP, Lazo G, Chao SM, Anderson OD, Linkiewicz AM, Dubcovsky J, La Rota M, Sorrells ME, Zhang DS, Nguyen HT, Kalavacharla V, Hossain K, Kianian SF, Peng JH, Lapitan NLV, Gonzalez-Hernandeiz JL, Anderson JA, Choi DW, Close TJ, Dilbirligi M, Gill KS, Walker-Simmons MK, Steber C, McGuire PE, Qualset CO, Dvorak J (2003) The organization and rate of evolution of wheat genomes are correlated with recombination rates along chromosome arms. Genome Res 13:753–763

    Article  PubMed  CAS  Google Scholar 

  • Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218

    CAS  Google Scholar 

  • Bennett MD, Smith JB (1976) Nuclear DNA amounts in angiosperms. Philos Trans R Soc Lond B 274:227–274

    CAS  Google Scholar 

  • Babb S, Muehlbauer GJ (2003) Genetic and morphological characterization of the barley uniculm2 (cul2) mutant. Theor Appl Genet 106:846–857

    PubMed  CAS  Google Scholar 

  • Buck-Sorlin GH (2002) The search for QTL in barley (Hordeum vulgare L.) using a new mapping population. Cell Mol Biol Lett 7:523–535

    PubMed  CAS  Google Scholar 

  • Dhaliwal HS, Multani DS, Sharma SK, Malkiat-Singh (1987) Induction of useful variability in Triticum monococcum L. Crop Improv 14(1):1–5

    Google Scholar 

  • Delaney DE, Nasuda S, Endo TR, Gill BS, Hulbert SH (1995) Cytologically based physical maps of the group-3 chromosomes of wheat. Theor Appl Genet 91:780–782

    CAS  Google Scholar 

  • Devos KM, Gale MD (2000) Genome relationships: the grass model in current research. Plant Cell 12(5):637–646

    Article  PubMed  CAS  Google Scholar 

  • Devos KM, Gale MD (1993a) Extended genetic maps of the homoeologous group 3 chromosomes of wheat, rye and barley. Theor Appl Genet 85(6–7):649–652

    CAS  Google Scholar 

  • Devos KM, Millan T, Gale MD (1993b) Comparative RFLP maps of the homoeologous group-2 chromosomes of wheat, rye and barley. Theor Appl Genet 85(6–7):784–792

    CAS  Google Scholar 

  • Devos KM, Atkinson MD, Chinoy CN, Francis HA, Harcourt RL, Koebner RMD, Liu CJ, Masojc P, Xie DX, Gale MD (1993c) Chromosomal rearrangements in the rye genome relative to that of wheat. Theor Appl Genet 85(6–7):673–680

    CAS  Google Scholar 

  • Devos KM, Wang ZM, Beales J, Sasaki T, Gale MD (1998) Comparative genetic maps of foxtail millet (Setaria italica) and rice (Oryza sativa). 96(1):63–68

  • Doebley J, Stec A, Hubbard L (1997) The evolution of apical dominance in maize. Nature 386:485–488

    Article  PubMed  CAS  Google Scholar 

  • Doust AN, Devos KM, Gadberry MD, Gale MD, Kellog EA (2005) Genetic control of branching in foxtail millet. Proc Natl Acad Sci USA 101(24):9045–9050

    Article  Google Scholar 

  • Dubcovsky J, Luo MC, Zhong GY, Bransteitter R, Desai A, Kilian A, Kleinhofs A, Dvorak J (1996) Genetic map of diploid wheat, Triticum monococcum L., and its comparison with maps of Hordeum vulgare L. Genetics 143:983–999

    PubMed  CAS  Google Scholar 

  • Dubcovsky J, Lijavetzky D, Appendino L, Tranquilli G (1998) Comparative RFLP mapping of Triticum monococcum genes controlling vernalization requirement. Theor Appl Genet 97(5/6):968–975

    Article  CAS  Google Scholar 

  • Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295–307

    CAS  Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction fragments to a high specific activity. Anal Biochem 132:6–13

    Article  PubMed  CAS  Google Scholar 

  • Feuillet C, Keller B (1999) High gene density is conserved at syntenic loci of small and large grass genomes. Proc Natl Acad Sci U S A 96:8265–8270

    Article  PubMed  CAS  Google Scholar 

  • Franckowiak JD (1996) Revised linkage maps for morphological markers in barley, Hordeum vulgare. Barley Genet Newsl 26:4

    Google Scholar 

  • Franckowiak JD, Lundqvist U (2002) Current list of new and revised barley genetic stock descriptions. Barley Genet Newsl 32:47 (http://www.wheat.pw.usda.gov/ggpages/bgn/32/BGS_Part1.html)

    Google Scholar 

  • Gallego F, Feuillet C, Messmer M, Penger A, Graner A, Yano M, Sasaki T, Keller B (1998) Comparative mapping of the two wheat leaf rust resistance loci Lr1 and Lr10 in rice and barley. Genome 41:328–336

    Article  PubMed  CAS  Google Scholar 

  • Gill KS, Gill BS, Endo TR, Boyko EV (1996) Identification and high-density mapping of gene-rich regions in chromosome group 5 of wheat. Genetics 143:1001–1012

    PubMed  CAS  Google Scholar 

  • Gupta K, Balyan S, Edwards J, Isaac P, Korzun V, Roder M, Gautier MF, Joudrier P, Schlatter R, Dubcovsky J, De La Pena C, Khairallah M, Penner G, Hayden J, Sharp P, Keller B, Wang C, Hardouin P, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422

    Article  PubMed  CAS  Google Scholar 

  • Guyomarc’h H, Sourdille P, Charmet G, Edwards J, Bernard M (2002a) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet 104:1164–1172

    Article  CAS  Google Scholar 

  • Guyomarc’h H, Sourdille P, Edwards J, Bernard M (2002b) Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons. Theor Appl Genet 105:736–744

    Article  CAS  Google Scholar 

  • Kato K, Miura H, Sawada S (2000) Mapping QTLs controlling grain yield and its components on chromosome 5A of wheat. Theor Appl Genet 101:1114–1121

    Article  CAS  Google Scholar 

  • Klages KH (1926) Metrical attributes and the physiology of hardy varieties of winter wheat. Agron J 18:529–566

    Article  Google Scholar 

  • Kosambi D (1944) Estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Kurata N, Moore G, Nagamura Y, Foote T, Yano M, Minobe Y, Gale M (1994) Conservation of genome structure between rice and wheat. Biotechnology 12:276–278

    Article  CAS  Google Scholar 

  • Lander E, Green P, Barlow A, Daley P, Stein L, et al (1987) MAPMAKER: an interactive computer package for constructing primary linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Li W, Gill BS (2004) Genomics for cereal improvement. In: Gupta PK, Varshney RK (eds) Cereal Genomics. Kuwer Academic Publishers pp 585–634

  • Li WL, Nelson JC, Chu CY, Shi LH, Huang SH, Liu DJ (2002) Chromosomal locations and genetic relationships of tiller and spike characters in wheat. Euphytica 125:357–366

    Article  CAS  Google Scholar 

  • Li X, Qian Q, Fu Z, Wang Y, Xiong G, Zeng D, Wang X, Liu X, Teng S, Hiroshi F, Yuan M, Luo D, Han B, Li J (2003) Control of tillering in rice. Nature 422:618–621

    Article  PubMed  CAS  Google Scholar 

  • Lijavetzky D, Muzzi G, Wicker T, Keller B, Wing R, Dubcovsky J (1999) Construction and characterization of a bacterial artificial chromosome (BAC) library for the A genome of wheat. Genome 42:1176–1182

    Article  PubMed  CAS  Google Scholar 

  • Liu S, Anderson JA (2003) Targeted molecular mapping of a major wheat QTL for Fusarium head blight resistance using wheat ESTs and synteny with rice. Genome 46:817–823

    Article  PubMed  CAS  Google Scholar 

  • Lukaszewski AJ (1992) A comparison of physical distribution of recombination in chromosome 1R in diploid rye and in hexaploid triticale. Theor Appl Genet 83(8):1048–1053

    Article  Google Scholar 

  • Lukaszewski AJ, Rybka K, Korzun V, Malyshev SV, Lapinski B, Whitkus R (2004) Genetic and physical mapping of homoeologous recombination points involving wheat chromosome 2B and rye chromosome 2R. 47(1):36–45

  • Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK (1999) The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene. Development 126:469–481

    PubMed  CAS  Google Scholar 

  • Malyshev S, Korzun V, Voylokov A, Smirnov V, Borner A (2001) Linkage mapping of mutant loci in rye (Secale cereale L.). Theor Appl Genet 103:70–74

    Article  CAS  Google Scholar 

  • McGuire PE, Qualset CO (1996) Progress in genome mapping of wheat and related species. Joint proceedings of the 5th and 6th public workshops of the International Triticeae Mapping Initiative (ITMI). Genetic Resource Conservation Program University of California, CA, pp 38–50

  • Otsuga D, DeGuzman B, Prigge MJ, Drews GN, Clark SE (2001) REVOLUTA regulates meristem initiation at lateral positions. Plant J 25:223–236

    Article  PubMed  CAS  Google Scholar 

  • Qi L, Echalier B, Friebe B, Gill BS (2003) Molecular characterization of a set of wheat deletion stocks for use in chromosome bin mapping of ESTs. Funct Integr Genomics 3:39–55

    PubMed  CAS  Google Scholar 

  • Richards RA (1988) A tiller inhibition gene in wheat and its effect on plant growth. Austr J Agric Res 39:749–757

    Article  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory press, Cold Spring Harbor

    Google Scholar 

  • Schmitz G, Tillmann E, Carriero F, Fiore C, Cellini F, Theres K (2002) The tomato Blind gene encodes a MYB transcription factor that controls the formation of lateral meristems. Proc Natl Acad Sci U S A 99:1064–1069

    Article  PubMed  CAS  Google Scholar 

  • Schumacher K, Schmitt T, Rossberg M, Schmitz G, Theres K (1999) The Lateral suppressor (Ls) gene of tomato encodes a new member of the VHIID protein family. Proc Natl Acad Sci USA 96:290–295

    Article  PubMed  CAS  Google Scholar 

  • Sears ER, Sears LMS (1978) The telocentric chromosomes of common wheat. In: Ramanujam S(ed) Proceedings of the 5th international wheat genetics symposium, Indian Society of Genetics and Plant Breeding, New Delhi, India, pp 29–45

  • Shah MM, Gill KS, Baenziger PS, Yen Y, Kaeppler SM Ariyarathne HM (1999) Molecular mapping of loci for agronomic traits on chromosome 3A of bread wheat. Crop Sci 39:1728–1732

    Article  CAS  Google Scholar 

  • Snape JW, Quarrie SA, Laurie DA (1996) Comparative mapping and its use for the genetic analysis of agronomic characters in wheat. Euphytica 89(1):27–31

    Article  CAS  Google Scholar 

  • Snowden KC, Simkin AJ, Janssen BJ, Templeton KR, Loucas HM, Simons JL, Karunairetnam S, Gleave AP, Clark DG, Klee HJ (2005) The Decreased apical dominance1/Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE8 gene affects branch production and plays a role in leaf senescence, root growth, and flower development. Plant Cell 17:746–759

    Article  PubMed  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Sorefan K, Booker J, Haurogne K, Goussot M, Bainbridge K, Foo E, Chatfield S, Ward S, Beveridge C, Rameau C, Leyser O (2003) MAX4 and RMS1 are orthologous dioxygenase-like genes that regulate shoot branching in Arabidopsis and pea. Genes Dev 17:1469–1474

    Article  PubMed  CAS  Google Scholar 

  • Sorrells ME, La Rota M, Bermudez-Kandianis CE, Greene RA, Kantety R, Munkvold JD, Miftahudin, Mahmoud A, Ma X, Gustafson PJ, Qi LL, Echalier B, Gill BS, Matthews DE, Lazo GR, Chao S, Anderson OD, Edwards H, Linkiewicz AM, Dubcovsky J, Akhunov ED, Dvorak J, Zhang D, Nguyen HT, Peng J, Lapitan NL, Gonzalez-Hernandez JL, Anderson JA, Hossain K, Kalavacharla V, Kianian SF, Choi DW, Close TJ, Dilbirligi M, Gill KS, Steber C, Walker-Simmons MK, McGuire PE, Qualset CO (2003) Comparative DNA sequence analysis of wheat and rice genomes. Genome Res 13:1818–1827

    PubMed  CAS  Google Scholar 

  • Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25

    Article  PubMed  CAS  Google Scholar 

  • Spielmeyer W, Richards RA (2004) Comparative mapping of wheat chromosome 1AS which contains the tiller inhibition gene (tin) with rice chromosome 5S. Theor Appl Genet 109:1303–1310

    Article  PubMed  CAS  Google Scholar 

  • Stirnberg P, van De Sande K, Leyser HM (2002) MAX1 and MAX2 control shoot lateral branching in Arabidopsis. Development 129:1131–1141

    PubMed  CAS  Google Scholar 

  • Tantikanjana T, Mikkelsen MD, Hussain M, Halkier BA, Sundaresan V (2004) Functional analysis of the tandem-duplicated P450 genes SPS/BUS/CYP79F1 and CYP79F2 in glucosinolate biosynthesis and plant development by Ds transposition-generated double mutants. Plant Physiol 135:840–848

    Article  PubMed  CAS  Google Scholar 

  • Tantikanjana T, Yong JW, Letham DS, Griffith M, Hussain M, Ljung K, Sandberg G, Sundaresan V (2001) Control of axillary bud initiation and shoot architecture in Arabidopsis through the SUPERSHOOT gene. Genes Dev 15:1577–1588

    Article  PubMed  CAS  Google Scholar 

  • Werner JE, Endo TR, Gill BS (1992) Toward a cytogenetically based physical map of the wheat genome. Proc Natl Acad Sci USA 89:11307–11311

    Article  PubMed  CAS  Google Scholar 

  • Wu WR, Li WM, Tang DZ, Lu HR, Worland AJ (1999) Time-related mapping of quantitative trait loci underlying tiller number in rice. Genetics 151:297–303

    PubMed  CAS  Google Scholar 

  • Van Deynze AE, Nelson JC, Yglesias ES, Harrington SE, Braga DP, McCouch SR, Sorrells ME (1995) Comparative mapping in grasses. Wheat relationships. Mol Gen Genet 248:744–754

    Article  PubMed  Google Scholar 

  • Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421

    Article  Google Scholar 

Download references

Acknowledgment

We thank Drs. M.D. Gale, A. Kleinhofs, and M.E. Sorrells for providing RFLP probes; and Guihua Bai and Gina Brown-Guedira for providing microsatellite markers. We extend our special thanks to Duane Wilson and John Raupp.for their excellent technical assistance. This paper is contribution number 07-16-J from the Kansas Agricultural Experiment Station.

Author information

Authors and Affiliations

  1. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506-5502, USA

    Vasu Kuraparthy, Shilpa Sood & Bikram S. Gill

  2. Department of Biotechnology, Indian Institute of Technology, Roorkee, 247667, Uttaranchal, India

    H. S. Dhaliwal

  3. Department of Plant Breeding, Genetics and Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, Punjab, India

    Parveen Chhuneja

Authors
  1. Vasu Kuraparthy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shilpa Sood
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. S. Dhaliwal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Parveen Chhuneja
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bikram S. Gill
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bikram S. Gill.

Additional information

Communicated by M. Xu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kuraparthy, V., Sood, S., Dhaliwal, H.S. et al. Identification and mapping of a tiller inhibition gene (tin3) in wheat. Theor Appl Genet 114, 285–294 (2007). https://doi.org/10.1007/s00122-006-0431-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-006-0431-y

Keywords

Search

Navigation