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Hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. suaveolens: evidence that the Q chromosome causes hybrid lethality based on Q-chromosome-specific DNA markers

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Abstract

Hybrid seedlings from the cross Nicotiana tabacum × N. suaveolens express lethality at 28°C. We carried out a cross between monosomic lines of N. tabacum lacking the Q chromosome and N. suaveolens by test-tube pollination and ovule culture at 28°C. To suppress hybrid lethality, hybrid seedlings obtained were transferred to 36°C immediately after germination and cultured. We determined whether Q-chromosome-specific DNA markers were detected among hybrid seedlings. When hybrid seedlings cultured at 36°C were transferred to 28°C, hybrid seedlings in which Q-chromosome-specific DNA markers were detected expressed hybrid lethality, while hybrid seedlings in which Q-chromosome-specific DNA markers were not detected did not express hybrid lethality. From these results, we concluded that the presence of the Q chromosome of N. tabacum is related to hybrid lethality observed in crosses between N. tabacum and N. suaveolens. This is the first report that clearly demonstrates the relationship between a certain chromosome and hybrid lethality in the genus Nicotiana using chromosome-specific DNA markers. Additionally, we confirmed that the Q chromosome belongs to the S subgenome because Q-chromosome-specific DNA markers were detected only in N. sylvestris.

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Acknowledgments

We thank Dr. G. Kokubugata (Tsukuba Botanical Garden, National Science Museum, Japan) for helpful discussions. This work was partly supported by Grant-in-Aid for Scientific Research (A) no. 13306003 and (C) no. 15580003 from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. School of Agriculture, Ibaraki University, 300-0393, Ami, Ibaraki, Japan

    T. Tezuka

  2. School of Agriculture, Meiji University, 214-8571, Kawasaki, Kanagawa, Japan

    W. Marubashi

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  1. T. Tezuka
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  2. W. Marubashi
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Correspondence to W. Marubashi.

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Communicated by J. S. Heslop-Harrison

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Tezuka, T., Marubashi, W. Hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. suaveolens: evidence that the Q chromosome causes hybrid lethality based on Q-chromosome-specific DNA markers. Theor Appl Genet 112, 1172–1178 (2006). https://doi.org/10.1007/s00122-006-0219-0

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  • DOI: https://doi.org/10.1007/s00122-006-0219-0

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