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Molecular mapping of Fusarium head blight resistance in the winter wheat population Dream/Lynx

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Abstract

Fusarium head blight (FHB), mainly caused by Fusarium graminearum and F. culmorum, can significantly reduce the grain quality of wheat (Triticum aestivum L.) due to mycotoxin contamination. The objective of this study was to identify quantitative trait loci (QTLs) for FHB resistance in a winter wheat population developed by crossing the resistant German cultivar Dream with the susceptible British cultivar Lynx. A total of 145 recombinant inbred lines (RILs) were evaluated following spray inoculation with a F. culmorum suspension in field trials in 2002 in four environments across Germany. Based on amplified fragment length polymorphism and simple sequence repeat marker data, a 1,734 cM linkage map was established assuming that the majority of the polymorphic parts of the genome were covered. The area under disease progress curve (AUDPC) was calculated based on the visually scored FHB symptoms. The population segregated quantitatively for FHB severity. Composite interval mapping analysis for means across the environments identified four FHB resistance QTLs on chromosomes 6AL, 1B, 2BL and 7BS. Individually the QTLs explained 19%, 12%, 11% and 21% of the phenotypic variance, respectively, and together accounted for 41%. The QTL alleles conferring resistance on 6AL, 2BL and 7BS originated from cv. Dream. The resistance QTL on chromosome 6AL partly overlapped with a QTL for plant height. The FHB resistance QTL on 7BS coincided with a QTL for heading date, but the additive effect on heading date was of minor importance. The resistance QTL on chromosome 1B was associated with the T1BL.1RS wheat-rye translocation of Lynx.

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Acknowledgements

Our special thanks for excellent technical assistance are extended to E. Madge-Pimentel, L. Logothetis and S. Schmidt and to the staff of the departments of Biotechnology and Wheat Breeding of the Bavarian State Research Centre. We would like to thank B. Schneider, M. Scholz and S. Sabrowski for evaluating the mapping population in the field trials in Hohenheim, Wetze and Wohlde. We are grateful to M. Roeder for the opportunity to screen our parental lines with SRR markers at the IPK Gatersleben. This project was supported by the German Federal Ministry of Education and Research, the Lochow-Petkus GmbH and the EUREKA Consortium (Project No. 2386).

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Authors and Affiliations

  1. Institute for Crop Production and Plant Breeding, Bavarian State Research Center for Agriculture, Am Gereuth 8, 85354, Freising, Germany

    M. Schmolke, G. Zimmermann, G. Schweizer & L. Hartl

  2. Department for Agrobiotechnology Tulln, University of Natural Resources and Applied Life Sciences, Konrad Lorenz Strasse 20, 3430, Tulln, Austria

    H. Buerstmayr

  3. State Plant Breeding Institute, University of Hohenheim, 70593, Stuttgart, Germany

    T. Miedaner

  4. Lochow-Petkus GmbH, Bollersener Weg 5, 29303, Bergen, Germany

    V. Korzun & E. Ebmeyer

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  1. M. Schmolke
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Correspondence to L. Hartl.

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Communicated by R. Bernardo

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Schmolke, M., Zimmermann, G., Buerstmayr, H. et al. Molecular mapping of Fusarium head blight resistance in the winter wheat population Dream/Lynx. Theor Appl Genet 111, 747–756 (2005). https://doi.org/10.1007/s00122-005-2060-2

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