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Genetic basis of barley caryopsis dormancy and seedling desiccation tolerance at the germination stage

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Abstract

The genomic regions controlling caryopsis dormancy and seedling desiccation tolerance were identified using 152 F4 lines derived from a cross between Mona, a Swedish cultivar, and an Israeli xeric wild barley Hordeum spontaneum genotype collected at Wadi Qilt, Israel. Dormancy, the inability of a viable seed to germinate, and desiccation tolerance, the ability of the desiccated seedlings to revive after rehydration, were characterized by fitting the germination and revival data with growth curves, using three parameters: minimum, maximum, and slope of germination or revival rate derived by the least square method. The genetic map was constructed with 85 genetic markers (SSRs, AFLPs, STSs, and Dhn genes) using the multipoint-mapping algorithm. Quantitative trait loci (QTLs) mapping was conducted with the multiqtl package. Ten genomic regions were detected that affected the target traits, seven of which affected both dormancy and desiccation tolerance traits. Both the wild barley genotype and the Swedish cultivar contributed the favorite alleles for caryopsis dormancy, whereas seedling desiccation tolerance was attributed to alleles descending from the cultivar. The results indicate that some barley dormancy genes are lost during domestication and that dormancy QTLs are associated with abiotic stress tolerance.

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Acknowledgements

This work was supported by the U.S. AID Cooperative Development Research Program (grant TA-MOU-97_CA17-001), German-Israeli Project Cooperation (grant DIP-B-4.3), the Israel Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Molecular Genetics and Evolution.

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Authors and Affiliations

  1. Institute of Forestry, Chinese Academy of Forestry, Haidian District, Beijing, 100091, China

    F. Zhang

  2. Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, 31905, Israel

    G. Chen, Q. Huang, O. Orion, T. Krugman, T. Fahima, A. B. Korol & E. Nevo

  3. Wyler Department of Dryland Agriculture, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boker Campus, Beer-Sheva, 84990, Israel

    Y. Gutterman

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  1. F. Zhang
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  2. G. Chen
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  3. Q. Huang
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  4. O. Orion
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  5. T. Krugman
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  6. T. Fahima
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  7. A. B. Korol
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  8. E. Nevo
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  9. Y. Gutterman
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Correspondence to Y. Gutterman.

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Communicated by R. Hagemann

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Zhang, F., Chen, G., Huang, Q. et al. Genetic basis of barley caryopsis dormancy and seedling desiccation tolerance at the germination stage. Theor Appl Genet 110, 445–453 (2005). https://doi.org/10.1007/s00122-004-1851-1

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  • DOI: https://doi.org/10.1007/s00122-004-1851-1

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