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A genetic analysis of adult plant resistance to stripe rust in the wheat cultivar Kariega

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Abstract

The wheat cultivar Kariega expresses complete adult plant resistance to stripe rust in South Africa. The aim of this investigation was to determine the extent and nature of variability in stripe rust resistance in a population of 150 doubled haploid lines generated from a cross between Kariega and the susceptible cultivar Avocet S. Analysis of field data for adult plant stripe rust resistance identified two major QTLs and two minor QTLs in the resistant cultivar Kariega. The two major QTLs were located on chromosomes 7D (QYr.sgi-7D) and 2B (QYr.sgi-2B.1), contributing 29% and 30% to the phenotypic variance, respectively. QYr.sgi-2B.1 is primarily associated with a chlorotic and/or necrotic response, unlike QYr.sgi-7D, which is believed to be the adult plant resistance gene Yr18. These two QTLs for adult plant resistance in Kariega appear to represent different forms of resistance, where QYr.sgi-7D may represent potentially more durable resistance than QYr.sgi-2B.1. Mixture model analysis of the field leaf infection scores suggested a genetic model involving two independent genes combining in a classical, epistatic manner. The results of the QTL analysis demonstrate its higher resolution power compared to the mixture model analysis by detecting the presence of minor QTLs.

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Acknowledgements

We thank C.M. Bender (UFS) for assistance in planting and F.J. Kloppers (PANNAR) for the trial site and field plot care. Financial assistance by the South African Winter Cereal Research Trust, Agricultural Research Council and National Research Foundation is also acknowledged. L.B. would like to thank the British Society of Plant Pathology for a fellowship supporting this collaboration.

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Authors and Affiliations

  1. ARC-Small Grain Institute, P.O. Box 3507, Matieland 7602, South Africa

    V. P. Ramburan & R. Prins

  2. Department of Plant Sciences, University of the Free State, P.O. Box 339, Bloemfontein 9300, South Africa

    Z. A. Pretorius

  3. Department of Genetics, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa

    V. P. Ramburan & J. H. Louw

  4. Department of Disease and Stress Biology, John Innes Centre, Norwich, NR47UH, UK

    L. A. Boyd & P. H. Smith

  5. ARC-Small Grain Institute, Private Bag X29, Bethlehem 9700, South Africa

    W. H. P. Boshoff

Authors
  1. V. P. Ramburan
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  2. Z. A. Pretorius
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  3. J. H. Louw
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  4. L. A. Boyd
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  5. P. H. Smith
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  6. W. H. P. Boshoff
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  7. R. Prins
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Correspondence to V. P. Ramburan.

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Communicated by P. Langridge

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Ramburan, V.P., Pretorius, Z.A., Louw, J.H. et al. A genetic analysis of adult plant resistance to stripe rust in the wheat cultivar Kariega. Theor Appl Genet 108, 1426–1433 (2004). https://doi.org/10.1007/s00122-003-1567-7

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  • DOI: https://doi.org/10.1007/s00122-003-1567-7

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