Abstract
The incompatibility genetics of sour cherry (Prunus cerasus), an allotetraploid species thought to be derived from sweet cherry (diploid) and ground cherry (tetraploid), were investigated by test crossing and by analysis of stylar ribonucleases which are known to be the products of incompatibility alleles in sweet cherry. Stylar extracts of 36 accessions of sour cherry were separated electrophoretically and stained for ribonuclease activity. The zymograms of most accessions showed three bands, some two or four. Of the ten bands seen, six co-migrated with bands that in sweet cherry are attributed to the incompatibility alleles S 1 , S 3 , S 4 , S 6, S 9 and S 13 . ‘Čačanski Rubin’, ‘Erdi Botermo B’, ‘Koroš’ and ‘Ujfehertoi Furtoš’, which showed bands apparently corresponding to S 1 and S 4 , were test pollinated with the sweet cherry ‘Merton Late’ (S 1 S 4 ). Monitoring pollen tube growth, and, in one case, fruit set, showed that these crosses were incompatible and that the four sour cherries indeed have the alleles S 1 and S 4 . Likewise, test pollination of ‘Marasca Piemonte’, ‘Marasca Savena’ and ‘Morello, Dutch’ with ‘Noble’ (S 6 S 13 ) showed that these three sour cherries have the alleles S 6 and S 13 . S 13 was very frequent in sour cherry cultivars, but is rare in sweet cherry cultivars, whereas with S 3 the situation is reversed. It was suggested that the other four bands are derived from ground cherry and one of these, provisionally attributed to S B , occurred frequently in a small set of ground cherry accessions surveyed. Analysing some progenies from sour by sweet crosses by S allele-specific PCR and monitoring the success of some sweet by sour crosses were informative. They indicated mostly disomic inheritance, with sweet cherry S alleles belonging to one locus and, presumably, the ground cherry alleles to the other, and helped clarify the genomic arrangement of the alleles and the interactions in heteroallelic pollen.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albertini A, Liverani A, Rivalta L, Cobianchi D (1988) Monographia di Cultivar di Ciliego Acido. Ministero dell’Agricoltura e delle Foreste, Rome
Apostol J (1996) Sour and sweet cherry breeding and production in Hungary. Acta Hortic 410:101–104
Beaver JA, Iezzoni AF (1993) Allozyme inheritance in tetraploid sour cherry (Prunus cerasus L.). J Am Soc Hortic Sci 118:873–877
Bošković R, Tobutt KR (1996) Correlation of stylar ribonuclease zymograms with incompatibility alleles in sweet cherry. Euphytica 90:245–250
Bošković R, Tobutt KR (2001) Analysing stylar ribonucleases to genotype cherry cultivars assigned to incompatibility groups. Theor Appl Genet 103:475–485
Bošković R, Russell K, Tobutt KR (1997) Inheritance of stylar ribonucleases in cherry progenies, and reassignment of incompatibility alleles to two incompatibility groups. Euphytica 95:221–228
Calabrese F, Fenech L, Raimondo A (1984) Kronio: una cultivar di ciliegio molto precoce e autocompatibile. Rivista di Frutticoltura e di Ortofloricoltura 46 (5):27–30
Cerović R (1992) Pollen tube growth in sour cherry pistils in relation to fruit set. Adv Hortic Sci 6:107–111
Crane MB (1927) Self and cross-sterility in fruit trees: a summary of results obtained from pollination experiments with plums, cherries and apples. J Pomol 6:157–166
Crane MB, Lawrence WJC (1929) Genetical and cytological aspects of incompatibility and sterility in cultivated fruits. J Pomol Hortic Sci 7:276–301
Crane MB, Lawrence WJC (1938) The genetics of garden plants. Macmillan, London
Darlington CD (1927) The behaviour of polyploids. Nature 119:390–391
De Vries DP (1968) Compatibility of cherries in The Netherlands. Euphytica 17:207–215
Hruby K (1963) Pollination interrelations in some sour cherry cultivars. Biol Plant 5:124–128
Iezzoni A, Schmidt H, Albertini A (1991) Cherries (Prunus). In: Moore JN, Ballington JR (eds) Genetic resources of temperate fruit and nut crops, vol 1. ISHS, Wageningen, The Netherlands, pp 111–173
Kao T, McCubbin AG (1996) How flowering plants discriminate between self and non-self pollen to prevent inbreeding. Proc Natl Acad Sci USA 93:12059–12065
Kobel F (1927) Zytologische Untersuchungen an Prunoideen und Pomoideen. Arch Julius Klaus-Stift Vererbforsch 3:1–84
Lansari A, Iezzoni A (1990) A preliminary analysis of self-incompatibility in sour cherry. HortScience 25:1636–1638
Lawrence WJC (1930) Incompatibility in polyploids. Genetica 12:269–296
Lewis D (1943) Physiology of incompatibility in plants III. Autopolyploids. J Genet 45:171–185
Lewis D (1947) Competition and dominance of incompatibility alleles in diploid pollen. Heredity 1:85–108
Lewis D (1954) Comparative incompatibility in angiosperms and fungi. Adv Genet 6:235–285
Lewis D, Modlibowska I (1942) Genetical studies in pear IV. Pollen tube growth and incompatibility. J Genet 43:211–222
Luu D-T, Qin X, Morse D, Cappadocia M (2000) S-RNase uptake by compatible pollen tubes in gametophytic self-incompatibility. Nature 407:649–651
Luu D-T, Qin X, Laublin G, Yang Q, Morse D, Cappadocia M (2001) Rejection of S-heteroallelic pollen by a dual-specific S-RNase in Solanum chacoense predicts a multimeric SI pollen component. Genetics 159:329–335
Matthews P, Dow KP (1969) Incompatibility groups: sweet cherry (Prunus avium). In: Knight RL (ed) Abstract bibliography of fruit breeding & genetics to 1965, Prunus. Commonwealth Agricultural Bureaux, Farnham Royal, pp 540–544
Montalti P, Selli R (1984) Osservazioni sulla fertilita del ciliegio acido. Riv Fruttic Ortofloric 46 (9/10): 57–62
Olden EJ, Nybom N (1968) On the origin of Prunus cerasus L. Hereditas 59:327–345
Preil W (1970) Observing the growth of pollen tubes in pistil and ovarian tissue by means of fluorescence microscopy. Zeiss Inf 75:24–25
Redalen G (1984a) Cross pollination of five sour cherry cultivars. Acta Hortic 149:71–76
Redalen G (1984b) Fertility in sour cherries. Gartenbauwissenschaft 49:212–217
Sonneveld T, Robbins TP, Bošković R, Tobutt KR (2001) Cloning of six cherry self-incompatibility alleles and development of allele-specific PCR detection. Theor Appl Genet 102:1046–1055
Sonneveld T, Tobutt KR, Robbins TP (2003) Allele-specific PCR detection of sweet cherry self-incompatibility (S) alleles S 1 to S 16 using consensus and allele-specific primers. Theor Appl Genet 107:1059–1070
Thompson RD, Kirch HH (1992) The S locus of flowering plants: when self-rejection is self-interest. Trends Genet 8:381–387
Tobutt KR, Bošković R, Sonneveld T (2001) Cherry (in)compatibility genotypes—harmonization of recent results from UK, Canada, Germany, Japan and USA. Eucarpia Fruit Breed Sec Newsl 5:41–46
Williams W, Brown AG (1956) Genetic response to selection in cultivated plants: gene frequencies in Prunus avium. Heredity 10:237–245
Yamane H, Tao R, Sugiura A, Hauck NR, Iezzoni AF (2001) Identification and characterization of S-RNases in tetraploid sour cherry (Prunus cerasus). J Am Soc Hortic Sci 126:661–667
Yenikeyev KK (1973) Development of self-fertile sour cherry varieties (C. vulgaris Mill.) in the central areas of the USSR. Atti Convegno del Ciliegio, Verona, June 1972:19–23
Acknowledgements
Cherry genetics at East Malling is supported by the Department for Environment, Food & Rural Affairs. Radovan Bošković acknowledges a grant from the Mount Trust and Tineke Sonneveld, a studentship from HRI and University of Nottingham. We thank Emma-Jane Lamont (Brogdale), Brigitte Wolfram (Dresden), Stein Harald Hjeltnes (Ullensvang), Henk Kemp (Wilhelminadorp) and Vlada Stevanović (Belgrade), for provision of plant material.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by H.F. Linskens
Rights and permissions
About this article
Cite this article
Tobutt, K.R., Bošković, R., Cerović, R. et al. Identification of incompatibility alleles in the tetraploid species sour cherry. Theor Appl Genet 108, 775–785 (2004). https://doi.org/10.1007/s00122-003-1511-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-003-1511-x