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Genetic mapping of Dn7, a rye gene conferring resistance to the Russian wheat aphid in wheat

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Abstract.

The Russian wheat aphid is a significant pest problem in wheat and barley in North America. Genetic resistance in wheat is the most effective and economical means to control the damage caused by the aphid. Dn7 is a rye gene located on chromosome 1RS that confers resistance to the Russian wheat aphid. The gene was previously transferred from rye into a wheat background via a 1RS/1BL translocation. This study was conducted to genetically map Dn7 and to characterize the type of resistance the gene confers. The resistant line '94M370' was crossed with a susceptible wheat cultivar that also contains a pair of 1RS/1BL translocation chromosomes. The F2 progeny from this cross segregated for resistance in a ratio of 3 resistant: 1 susceptible, indicating a single dominant gene. One-hundred and eleven RFLP markers previously mapped on wheat chromosomes 1A, 1B and 1D, barley chromosome 1H and rye chromosome 1R, were used to screen the parents for polymorphism. A genetic map containing six markers linked to Dn7, encompassing 28.2 cM, was constructed. The markers flanking Dn7 were Xbcd1434 and XksuD14, which mapped 1.4 cM and 7.4 cM from Dn7, respectively. Dn7 confers antixenosis, and provides a higher level of resistance than that provided by Dn4. The applications of Dn7 and the linked markers in wheat breeding are discussed.

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Acknowledgements.

We thank Frank Peairs and Jeff Rudolph for assistance with the screening of genetic materials with the Russian wheat aphid at the CSU Insectary. This project was partially funded by USDA Contract No. 98-34205-6375, USDA Contract No. 2001-52100-11293, and Hatch Funds.

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Author notes

  1. Garret R. Anderson

    Present address: Present addresses: University of Colorado Health Sciences Center, 4200 E. Ninth Avenue Box No. B146, Denver, CO 80262, USA,

Authors and Affiliations

  1. Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, USA

    Garret R. Anderson, Dan Papa, Junhua Peng, M. Tahir & Nora L. V. Lapitan

  2. Kansas Cancer Center, University of Kansas Medical Center, Kansas City, KS 66160, USA

    Dan Papa

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  1. Garret R. Anderson
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Correspondence to Nora L. V. Lapitan.

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Communicated by J. Dvorak

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Anderson, G.R., Papa, D., Peng, J. et al. Genetic mapping of Dn7, a rye gene conferring resistance to the Russian wheat aphid in wheat. Theor Appl Genet 107, 1297–1303 (2003). https://doi.org/10.1007/s00122-003-1358-1

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  • DOI: https://doi.org/10.1007/s00122-003-1358-1

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