Skip to main content
SpringerLink
Log in

Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract.

A genetic map was constructed with specific PCRs, DALPs and AFLPs using F8-generation sunflower recombinant inbred lines. RI lines generated from a F2 population of one cross between the two cultivated inbred lines HA89 (maintainer for Pet1 CMS) and LR4 (restorer for Pet1 CMS) were used. A total of 305 markers were located using seven sPCR, 64 DALP and 301 AFLP loci. They were generated with one, seven and 14 primer pairs, respectively. The map construction consisted of a two-step strategy using 6 and 3.1 LOD scores revealed by a simulation file. Mapped markers were assembled into 18 linkage groups covering 2,168.6 cM with an average of 6.1 cM. The distribution of DALPs and AFLPs revealed that both markers tagged different regions to enable covering most of the sunflower genome. This leads to the longest map published so far for sunflower.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. INRA, UR-Génétique et Amélioration des plantes, UMR-DGPC, 2 place P Viala, 34060 Montpellier cedex 1, France, , , , ,

    K. Langar,  Y. Griveau &  A. Bervillé

  2. Institut National Polytechnique - ENSA de Toulouse, Place des Hauts-Murats, BP 354 - F 31006 Toulouse Cedex 6, France, , , , ,

    L. Gentzbittel

  3. Centre IRD de Montpellier, 911 Av Agropolis, BP 64501, 34394 Montpellier CEDEX 5, France, , , , ,

    M. Lorieux

  4. Laboratoire de Génome, Populations et interactions CNRS UMR 5000, Université Montpellier 2 CC. 63, Place E. Bataillon, 34095 Montpellier, France, , , , ,

    E. Desmarais

Authors
  1. K. Langar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Lorieux
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Desmarais
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Griveau
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Gentzbittel
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Bervillé
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Electronic Publication

Rights and permissions

Reprints and permissions

About this article

Cite this article

Langar, .K., Lorieux, .M., Desmarais, .E. et al. Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines. Theor Appl Genet 106, 1068–1074 (2003). https://doi.org/10.1007/s00122-002-1087-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-002-1087-x

Search

Navigation