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Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe toxica in narrow-leafed lupin (Lupinus angustifolius L.)

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Abstract.

Phomopsis stem blight (PSB) caused by Diaporthe toxica is a major disease in narrow-leafed lupin (Lupinus angustifolius L.). The F2 progeny and the parental plants from a cross between a breeding line 75A:258 (containing a single dominant resistance gene Phr1 against the disease) and a commercial cultivar Unicrop (susceptible to the disease) were used for development of molecular markers linked to the disease resistance gene. Two pairs of co-dominant DNA polymorphisms were detected using the microsatellite-anchored fragment length polymorphism (MFLP) technique. Both pairs of polymorphisms were isolated from the MFLP gels, re-amplified by PCR, sequenced, and converted into co-dominant, sequence-specific and PCR-based markers. Linkage analysis by MAPMAKER suggested that one marker (Ph258M2) was 5.7 centiMorgans (cM) from Phr1, and the other marker (Ph258M1) was 2.1 cM from Ph258M2 but further away from Phr1. These markers are suitable for marker-assisted selection (MAS) in lupin breeding.

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Authors and Affiliations

  1. Crop Improvement Institute, Department of Agriculture Western Australia, Locked Bag No. 4, Bentley Delivery Center 6983, WA 6983, Australia, , , , ,

    H. Yang,  B. Buirchell &  M. Sweetingham

  2. Centre for Legumes in Mediterranean Agriculture, The University of Western Australia, Nedlands, WA 6009, Australia, , , , ,

    M. Shankar

  3. Servicio de Investigacion y Tecnologia Agraria, Junta de Castilla y Leon Ctra. Burgos-Portugal Km 118, Apdo 172, 47080 Valladolid, Spain, , , , ,

    C. Caminero

  4. Department of Botany, The University of Western Australia, Nedlands, WA 6009, Australia, , , , ,

    P. Smith

Authors
  1. H. Yang
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  2. M. Shankar
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  3. B. Buirchell
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  4. M. Sweetingham
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  5. C. Caminero
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  6. P. Smith
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Yang, .H., Shankar, .M., Buirchell, .B. et al. Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe toxica in narrow-leafed lupin (Lupinus angustifolius L.). Theor Appl Genet 105, 265–270 (2002). https://doi.org/10.1007/s00122-002-0925-1

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  • DOI: https://doi.org/10.1007/s00122-002-0925-1

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